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pHAGE_puro 质粒 DNA

发布时间：2025/4/1 11:07:31 阅读次数：32

CAT#:JW-1-10199 常温运输及保存

pHAGE_puro 质粒 DNA
pHAGE_puro Plasmid DNA
使用手册 V1.0

产品及特点
pHAGE_puro 是一种慢病毒类型的载体。pHAGE_puro 质粒大小为 7585 bp ，属于高拷贝数质粒，并配备了 CMV 启动子。pHAGE_puro 具有多克隆位点，便于外源基因的插入；同时，它还带有嘌呤霉素作为筛选标记。本质粒携带氨苄青霉素抗性基因。其图谱如下：

规格及成分
本产品使用塑料袋包装

成分            编号        规格    包装
pHAGE_puro 质粒 DNA 干粉    1-10199        2 μg    0.5 mL 本色管
使用手册            sc1-10199        1份    无

运输及保存    常温运输，溶解后需-20℃保存，有效期两年。
使用方法
使用前将本产品短暂离心后加入 100 μL 自备的超纯水或 TE 缓冲液溶解，可以得到 20 ng/μL 的质粒 DNA 溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒 DNA 进行各种后续实验。

DNA 序列
1 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG
61 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA
121 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT
181 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG
241 CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC
301 GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA GCCCAAGCTT
361ACCATGACCG    AGTACAAGCC    CACGGTGCGC    CTCGCCACCC    GCGACGACGT    CCCCAGGGCC
421GTACGCACCC    TCGCCGCCGC    GTTCGCCGAC    TACCCCGCCA    CGCGCCACAC    CGTCGATCCG
481GACCGCCACA    TCGAGCGGGT    CACCGAGCTG    CAAGAACTCT    TCCTCACGCG    CGTCGGGCTC
541GACATCGGCA    AGGTGTGGGT    CGCGGACGAC    GGCGCCGCGG    TGGCGGTCTG    GACCACGCCG
601GAGAGCGTCG    AAGCGGGGGC    GGTGTTCGCC    GAGATCGGCC    CGCGCATGGC    CGAGTTGAGC
661GGTTCCCGGC    TGGCCGCGCA    GCAACAGATG    GAAGGCCTCC    TGGCGCCGCA    CCGGCCCAAG
721GAGCCCGCGT    GGTTCCTGGC    CACCGTCGGC    GTCTCGCCCG    ACCACCAGGG    CAAGGGTCTG
781GGCAGCGCCG    TCGTGCTCCC    CGGAGTGGAG    GCGGCCGAGC    GCGCCGGGGT    GCCCGCCTTC
841CTGGAGACCT    CCGCGCCCCG    CAACCTCCCC    TTCTACGAGC    GGCTCGGCTT    CACCGTCACC
901GCCGACGTCG    AGGTGCCCGA    AGGACCGCGC    ACCTGGTGCA    TGACCCGCAA    GCCCGGTGCC
961TGAATCGATA    GATCCTAATC    AACCTCTGGA    TTACAAAATT    TGTGAAAGAT    TGACTGGTAT
1021TCTTAACTAT    GTTGCTCCTT    TTACGCTATG    TGGATACGCT    GCTTTAATGC    CTTTGTATCA
1081TGCTATTGCT    TCCCGTATGG    CTTTCATTTT    CTCCTCCTTG    TATAAATCCT    GGTTGCTGTC
1141TCTTTATGAG    GAGTTGTGGC    CCGTTGTCAG    GCAACGTGGC    GTGGTGTGCA    CTGTGTTTGC
1201TGACGCAACC    CCCACTGGTT    GGGGCATTGC    CACCACCTGT    CAGCTCCTTT    CCGGGACTTT
1261CGCTTTCCCC    CTCCCTATTG    CCACGGCGGA    ACTCATCGCC    GCCTGCCTTG    CCCGCTGCTG
1321GACAGGGGCT    CGGCTGTTGG    GCACTGACAA    TTCCGTGGTG    TTGTCGGGGA    AATCATCGTC
1381CTTTCCTTGG    CTGCTCGCCT    GTGTTGCCAC    CTGGATTCTG    CGCGGGACGT    CCTTCTGCTA
1441CGTCCCTTCG    GCCCTCAATC    CAGCGGACCT    TCCTTCCCGC    GGCCTGCTGC    CGGCTCTGCG
1501GCCTCTTCCG    CGTCTTCGCC    TTCGCCCTCA    GACGAGTCGG    ATCTCCCTTT    GGGCCGCCTC
1561CCCGCCTGAG    ATCCTTTAAG    ACCAATGACT    TACAAGGCAG    CTGTAGATCT    TAGCCACTTT
1621TTAAAAGAAA    AGGGGGGACT    GGAAGGGCTA    ATTCACTCCC    AACGAAGACA    AGATCTGCTT
1681TTTGCTTGTA    CTGGGTCTCT    CTGGTTAGAC    CAGATCTGAG    CCTGGGAGCT    CTCTGGCTAA
1741CTAGGGAACC    CACTGCTTAA    GCCTCAATAA    AGCTTGCCTT    GAGTGCTTCA    AGTAGTGTGT
1801GCCCGTCTGT    TGTGTGACTC    TGGTAACTAG    AGATCCCTCA    GACCCTTTTA    GTCAGTGTGG
1861AAAATCTCTA    GCAGTAGTAG    TTCATGTCAT    CTTATTATTC    AGTATTTATA    ACTTGCAAAG
1921AAATGAATAT    CAGAGAGTGA    GAGGCCCGGG    TTAATTAAGG    AAAGGGCTAG    ATCATTCTTG
1981AAGACGAAAG    GGCCTCGTGA    TACGCCTATT    TTTATAGGTT    AATGTCATGA    TAATAATGGT
2041TTCTTAGACG    TCAGGTGGCA    CTTTTCGGGG    AAATGTGCGC    GGAACCCCTA    TTTGTTTATT
2101TTTCTAAATA    CATTCAAATA    TGTATCCGCT    CATGAGACAA    TAACCCTGAT    AAATGCTTCA
2161ATAATATTGA    AAAAGGAAGA    GTATGAGTAT    TCAACATTTC    CGTGTCGCCC    TTATTCCCTT
2221TTTTGCGGCA    TTTTGCCTTC    CTGTTTTTGC    TCACCCAGAA    ACGCTGGTGA    AAGTAAAAGA
2281TGCTGAAGAT    CAGTTGGGTG    CACGAGTGGG    TTACATCGAA    CTGGATCTCA    ACAGCGGTAA
2341GATCCTTGAG    AGTTTTCGCC    CCGAAGAACG    TTTTCCAATG    ATGAGCACTT    TTAAAGTTCT
2401GCTATGTGGC    GCGGTATTAT    CCCGTGTTGA    CGCCGGGCAA    GAGCAACTCG    GTCGCCGCAT
2461ACACTATTCT    CAGAATGACT    TGGTTGAGTA    CTCACCAGTC    ACAGAAAAGC    ATCTTACGGA
2521TGGCATGACA    GTAAGAGAAT    TATGCAGTGC    TGCCATAACC    ATGAGTGATA    ACACTGCGGC
2581CAACTTACTT    CTGACAACGA    TCGGAGGACC    GAAGGAGCTA    ACCGCTTTTT    TGCACAACAT
2641GGGGGATCAT    GTAACTCGCC    TTGATCGTTG    GGAACCGGAG    CTGAATGAAG    CCATACCAAA
2701CGACGAGCGT    GACACCACGA    TGCCTGTAGC    AATGGCAACA    ACGTTGCGCA    AACTATTAAC
2761TGGCGAACTA    CTTACTCTAG    CTTCCCGGCA    ACAATTAATA    GACTGGATGG    AGGCGGATAA
2821AGTTGCAGGA    CCACTTCTGC    GCTCGGCCCT    TCCGGCTGGC    TGGTTTATTG    CTGATAAATC
2881TGGAGCCGGT    GAGCGTGGGT    CTCGCGGTAT    CATTGCAGCA    CTGGGGCCAG    ATGGTAAGCC
2941CTCCCGTATC    GTAGTTATCT    ACACGACGGG    GAGTCAGGCA    ACTATGGATG    AACGAAATAG
3001ACAGATCGCT    GAGATAGGTG    CCTCACTGAT    TAAGCATTGG    TAACTGTCAG    ACCAAGTTTA
3061CTCATATATA    CTTTAGATTG    ATTTAAAACT    TCATTTTTAA    TTTAAAAGGA    TCTAGGTGAA
3121GATCCTTTTT    GATAATCTCA    TGACCAAAAT    CCCTTAACGT    GAGTTTTCGT    TCCACTGAGC
3181GTCAGACCCC    GTAGAAAAGA    TCAAAGGATC    TTCTTGAGAT    CCTTTTTTTC    TGCGCGTAAT
3241CTGCTGCTTG    CAAACAAAAA    AACCACCGCT    ACCAGCGGTG    GTTTGTTTGC    CGGATCAAGA
3301GCTACCAACT    CTTTTTCCGA    AGGTAACTGG    CTTCAGCAGA    GCGCAGATAC    CAAATACTGT
3361TCTTCTAGTG    TAGCCGTAGT    TAGGCCACCA    CTTCAAGAAC    TCTGTAGCAC    CGCCTACATA
3421CCTCGCTCTG    CTAATCCTGT    TACCAGTGGC    TGCTGCCAGT    GGCGATAAGT    CGTGTCTTAC
3481CGGGTTGGAC    TCAAGACGAT    AGTTACCGGA    TAAGGCGCAG    CGGTCGGGCT    GAACGGGGGG
3541TTCGTGCACA    CAGCCCAGCT    TGGAGCGAAC    GACCTACACC    GAACTGAGAT    ACCTACAGCG
3601TGAGCTATGA    GAAAGCGCCA    CGCTTCCCGA    AGGGAGAAAG    GCGGACAGGT    ATCCGGTAAG
3661CGGCAGGGTC    GGAACAGGAG    AGCGCACGAG    GGAGCTTCCA    GGGGGAAACG    CCTGGTATCT
3721TTATAGTCCT    GTCGGGTTTC    GCCACCTCTG    ACTTGAGCGT    CGATTTTTGT    GATGCTCGTC
3781AGGGGGGCGG    AGCCTATGGA    AAAACGCCAG    CAACGCGGCC    TTTTTACGGT    TCCTGGCCTT
3841TTGCTGGCCT    TTTGCTCACA    TGTTCTTTCC    TGCGTTATCC    CCTGATTCTG    TGGATAACCG
3901TATTACCGCC    TTTGAGTGAG    CTGATACCGC    TCGCCGCAGC    CGAACGACCG    AGCGCAGCGA
3961GTCAGTGAGC    GAGGAAGCGG    AAGAGCGCCC    AATACGCAAA    CCGCCTCTCC    CCGCGCGTTG
4021GCCGATTCAT    TAATGCAGCA    AGCTCATGGC    TGACTAATTT    TTTTTATTTA    TGCAGAGGCC
4081GAGGCCGCCT    CGGCCTCTGA    GCTATTCCAG    AAGTAGTGAG    GAGGCTTTTT    TGGAGGCCTA
4141GGCTTTTGCA    AAAAGCTCCC    CGTGGCACGA    CAGGTTTCCC    GACTGGAAAG    CGGGCAGTGA
4201GCGCAACGCA    ATTAATGTGA    GTTAGCTCAC    TCATTAGGCA    CCCCAGGCTT    TACACTTTAT
4261GCTTCCGGCT    CGTATGTTGT    GTGGAATTGT    GAGCGGATAA    CAATTTCACA    CAGGAAACAG
4321CTATGACATG    ATTACGAATT    TCACAAATAA    AGCATTTTTT    TCACTGCATT    CTAGTTGTGG
4381TTTGTCCAAA    CTCATCAATG    TATCTTATCA    TGTCTGGATC    AACTGGATAA    CTCAAGCTAA
4441CCAAAATCAT    CCCAAACTTC    CCACCCCATA    CCCTATTACC    ACTGCCAATT    ACCTGTGGTT
4501TCATTTACTC    TAAACCTGTG    ATTCCTCTGA    ATTATTTTCA    TTTTAAAGAA    ATTGTATTTG
4561TTAAATATGT    ACTACAAACT    TAGTAGTTGG    AAGGGCTAAT    TCACTCCCAA    AGAAGACAAG
4621ATATCCTTGA    TCTGTGGATC    TACCACACAC    AAGGCTACTT    CCCTGATTAG    CAGAACTACA
4681CACCAGGGCC    AGGGGTCAGA    TATCCACTGA    CCTTTGGATG    GTGCTACAAG    CTAGTACCAG
4741TTGAGCCAGA    TAAGGTAGAA    GAGGCCAATA    AAGGAGAGAA    CACCAGCTTG    TTACACCCTG
4801TGAGCCTGCA    TGGGATGGAT    GACCCGGAGA    GAGAAGTGTT    AGAGTGGAGG    TTTGACAGCC
4861GCCTAGCATT    TCATCACGTG    GCCCGAGAGC    TGCATCCGGA    GTACTTCAAG    AACTGCTGAT
4921ATCGAGCTTG    CTACAAGGGA    CTTTCCGCTG    GGGACTTTCC    AGGGAGGCGT    GGCCTGGGCG
4981GGACTGGGGA    GTGGCGAGCC    CTCAGATCCT    GCATATAAGC    AGCTGCTTTT    TGCCTGTACT
5041GGGTCTCTCT    GGTTAGACCA    GATCTGAGCC    TGGGAGCTCT    CTGGCTAACT    AGGGAACCCA
5101CTGCTTAAGC    CTCAATAAAG    CTTGCCTTGA    GTGCTTCAAG    TAGTGTGTGC    CCGTCTGTTG
5161TGTGACTCTG    GTAACTAGAG    ATCCCTCAGA    CCCTTTTAGT    CAGTGTGGAA    AATCTCTAGC
5221AGTGGCGCCC    GAACAGGGAC    TTGAAAGCGA    AAGGGAAACC    AGAGGAGCTC    TCTCGACGCA
5281GGACTCGGCT    TGCTGAAGCG    CGCACGGCAA    GAGGCGAGGG    GCGGCGACTG    GTGAGTACGC
5341CAAAAATTTT    GACTAGCGGA    GGCTAGAAGG    AGAGAGATGG    GTGCGAGAGC    GTCAGTATTA
5401AGCGGGGGAG    AATTAGATCG    CGATGGGAAA    AAATTCGGTT    AAGGCCAGGG    GGAAAGAAAA
5461AATATAAATT    AAAACATATA    GTATGGGCAA    GCAGGGAGCT    AGAACGATTC    GCAGTTAATC
5521CTGGCCTGTT    AGAAACATCA    GAAGGCTGTA    GACAAATACT    GGGACAGCTA    CAACCATCCC
5581TTCAGACAGG    ATCAGAAGAA    CTTAGATCAT    TATATAATAC    AGTAGCAACC    CTCTATTGTG
5641TGCATCAAAG    GATAGAGATA    AAAGACACCA    AGGAAGCTTT    AGACAAGATA    GAGGAAGAGC
5701AAAACAAAAG    TAAGACCACC    GCACAGCAAG    CGGCCGGCCG    CTGATCTTCA    GACCTGGAGG
5761AGGAGATATG    AGGGACAATT    GGAGAAGTGA    ATTATATAAA    TATAAAGTAG    TAAAAATTGA
5821ACCATTAGGA    GTAGCACCCA    CCAAGGCAAA    GAGAAGAGTG    GTGCAGAGAG    AAAAAAGAGC
5881AGTGGGAATA    GGAGCTTTGT    TCCTTGGGTT    CTTGGGAGCA    GCAGGAAGCA    CTATGGGCGC
5941AGCGTCAATG    ACGCTGACGG    TACAGGCCAG    ACAATTATTG    TCTGGTATAG    TGCAGCAGCA
6001 GAACAATTTG    CTGAGGGCTA    TTGAGGCGCA    ACAGCATCTG    TTGCAACTCA    CAGTCTGGGG
6061 CATCAAGCAG    CTCCAGGCAA    GAATCCTGGC    TGTGGAAAGA    TACCTAAAGG    ATCAACAGCT
6121 CCTGGGGATT    TGGGGTTGCT    CTGGAAAACT    CATTTGCACC    ACTGCTGTGC    CTTGGAATGC
6181 TAGTTGGAGT    AATAAATCTC    TGGAACAGAT    TTGGAATCAC    ACGACCTGGA    TGGAGTGGGA
6241 CAGAGAAATT    AACAATTACA    CAAGCTTAAT    ACACTCCTTA    ATTGAAGAAT    CGCAAAACCA
6301 GCAAGAAAAG    AATGAACAAG    AATTATTGGA    ATTAGATAAA    TGGGCAAGTT    TGTGGAATTG
6361 GTTTAACATA    ACAAATTGGC    TGTGGTATAT    AAAATTATTC    ATAATGATAG    TAGGAGGCTT
6421 GGTAGGTTTA    AGAATAGTTT    TTGCTGTACT    TTCTATAGTG    AATAGAGTTA    GGCAGGGATA
6481 TTCACCATTA    TCGTTTCAGA    CCCACCTCCC    AACCCCGAGG    GGACCCGACA    GGCCCGAAGG
6541 AATAGAAGAA    GAAGGTGGAG    AGAGAGACAG    AGACAGATCC    ATTCGATTAG    TGAACGGATC
6601 TCGACGGTAT    CGCCGAATTC    ACAAATGGCA    GTATTCATCC    ACAATTTTAA    AAGAAAAGGG
6661 GGGATTGGGG    GGTACAGTGC    AGGGGAAAGA    ATAGTAGACA    TAATAGCAAC    AGACATACAA
6721 ACTAAAGAAT    TACAAAAACA    AATTACAAAA    ATTCAAAATT    TTCGGGTTTA    TTACAGGGAC
6781 AGCAGAGATC    CAGTTTGGAC    TAGTGGAGTT    CCGCGTTACA    TAACTTACGG    TAAATGGCCC
6841 GCCTGGCTGA    CCGCCCAACG    ACCCCCGCCC    ATTGACGTCA    ATAATGACGT    ATGTTCCCAT
6901 AGTAACGCCA    ATAGGGACTT    TCCATTGACG    TCAATGGGTG    GAGTATTTAC    GGTAAACTGC
6961 CCACTTGGCA    GTACATCAAG    TGTATCATAT    GCCAAGTACG    CCCCCTATTG    ACGTCAATGA
7021 CGGTAAATGG    CCCGCCTGGC    ATTATGCCCA    GTACATGACC    TTATGGGACT    TTCCTACTTG
7081 GCAGTACATC    TACGTATTAG    TCATCGCTAT    TACCATGGTG    ATGCGGTTTT    GGCAGTACAT
7141 CAATGGGCGT    GGATAGCGGT    TTGACTCACG    GGGATTTCCA    AGTCTCCACC    CCATTGACGT
7201 CAATGGGAGT    TTGTTTTGGC    ACCAAAATCA    ACGGGACTTT    CCAAAATGTC    GTAACAACTC
7261 CGCCCCATTG    ACGCAAATGG    GCGGTAGGCG    TGTACGGTGG    GAGGTCTATA    TAAGCAGAGC
7321 TCGTTTAGTG    AACCGTCAGA    TCGCCTGGAG    ACGCCATCCA    CGCTGTTTTG    ACCTCCATAG
7381 AAGACACCGG    CGGCCGCACC    GGTCTGCAGC    TAGCTCGAGA    ATTCTACCGG    GTAGGGGAGG
7441 CGCTTTTCCC    AAGGCAGTCT    GGAGCATGCG    CTTTAGCAGC    CCCGCTGGGC    ACTTGGCGCT
7501 ACACAAGTGG    CCTCTGGCCT    CGCACACATT    CCACATCCAC    CGGTAGGCGC    CAACCGGCTC
7561 CGTTCTTTGG    TGGCCCCTTC    GCGCC
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