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重组SARS冠状病毒2核衣壳蛋白

发布时间：2025/7/15 9:27:47 阅读次数：16

产品编号：JW-41417P
英文名称：Recombinant SARS-Cov-2 N protein, N-His
中文名称：重组SARS冠状病毒2核衣壳蛋白
英文别名：SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein. ;Recombinant SARS-Cov-2 N protein, His
性状：Lyophilized or Liquid
纯化方法：AC
理论分子量：46
检测分子量：50 kDa
浓度：＞0.5 mg/ml
储存液：PBS (pH7.4) with 0.02% Proclin300.
研究领域：Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus
亚基：Spike glycoprotein:
Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32155444, PubMed:32075877). The resulting peplomers protrude from the virus surface as spikes (By similarity).
Interacts with the accessory proteins 3a and 7a.
Spike protein S1:
Binds to human ACE2.
亚细胞定位：Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;
Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
组织特异性：The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.
Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444).
Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface.
翻译后修饰：The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.
Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444).
Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface.
活性：Not tested
序列：1-419/419
标签：N-His
纯度：>95% as determined by SDS-PAGE
内毒素：Not analyzed
表达系统：E.coli
复溶：Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
功能：attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
保存条件：Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事项：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.