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pPIC9K质粒DNA说明书

发布时间：2025/7/23 15:40:56 阅读次数：122

CAT#:1-5500
常温运输及保存

pPIC9K质粒DNA

产品及特点
本产品为pPIC9K质粒DNA干粉，是分泌型酵母表达载体，携带氨苄青霉素及卡那霉素抗性抗性基因。其图谱如下：

规格及成分
本产品使用塑料袋包装
成分            编号        规格    包装
pPIC9K质粒DNA干粉        1-5500        2 mg    0.5 mL本色管
使用手册            1-5500sc        1份    1份

运输及保存：常温运输，溶解后需-20℃保存，有效期两年。
使用方法：使用前将本产品短暂离心后加入100 μL自备超纯水或TE缓冲液溶解，得到20 ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。
DNA序列

1        CAGAGATTTT GAGACACAAC GTGGCTTTCC CCCCCCCCCT GCAGGTCGGC ATCACCGGCG
61       CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC
121      GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG
181      GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG
241      GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG
301      TATCTATGAT TGGAAGTATG GGAATGGTGA TACCCGCATT CTTCAGTGTC TTGAGGTCTC
361      CTATCAGATT ATGCCCAACT AAAGCAACCG GAGGAGGAGA TTTCATGGTA AATTTCTCTG
421      ACTTTTGGTC ATCAGTAGAC TCGAACTGTG AGACTATCTC GGTTATGACA GCAGAAATGT
481      CCTTCTTGGA GACAGTAAAT GAAGTCCCAC CAATAAAGAA ATCCTTGTTA TCAGGAACAA
541      ACTTCTTGTT TCGAACTTTT TCGGTGCCTT GAACTATAAA ATGTAGAGTG GATATGTCGG
601      GTAGGAATGG AGCGGGCAAA TGCTTACCTT CTGGACCTTC AAGAGGTATG TAGGGTTTGT
661      AGATACTGAT GCCAACTTCA GTGACAACGT TGCTATTTCG TTCAAACCAT TCCGAATCCA
721      GAGAAATCAA AGTTGTTTGT CTACTATTGA TCCAAGCCAG TGCGGTCTTG AAACTGACAA
781      TAGTGTGCTC GTGTTTTGAG GTCATCTTTG TATGAATAAA TCTAGTCTTT GATCTAAATA
841      ATCTTGACGA GCCAGACGAT AATACCAATC TAAACTCTTT AAACGTTAAA GGACAAGTAT
901      GTCTGCCTGT ATTAAACCCC AAATCAGCTC GTAGTCTGAT CCTCATCAAC TTGAGGGGCA
961      CTATCTTGTT TTAGAGAAAT TTGCGGAGAT GCGATATCGA GAAAAAGGTA CGCTGATTTT
1021     AAACGTGAAA TTTATCTCAA GATCTGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
1081     CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
1141     AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC
1201     CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG
1261     TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC
1321     GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
1381     GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
1441     ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
1501     CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
1561     CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
1621     GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
1681     TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
1741     AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
1801     CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
1861     CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
1921     TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
1981     TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
2041     CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
2101     AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
2161     AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
2221     AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT
2281     GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT
2341     GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG
2401     CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
2461     CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA
2521     ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG
2581     CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG
2641     GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT
2701     CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
2761     TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG
2821     GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC
2881     CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG
2941     GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
3001     TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
3061     GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
3121     GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC
3181     TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA
3241     CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT
3301     ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA ATTAATTCTC ATGTTTGACA
3361     GCTTATCATC GATAAGCTGA CTCATGTTGG TATTGTGAAA TAGACGCAGA TCGGGAACAC
3421     TGAAAAATAA CAGTTATTAT TCGAGATCTA ACATCCAAAG ACGAAAGGTT GAATGAAACC
3481     TTTTTGCCAT CCGACATCCA CAGGTCCATT CTCACACATA AGTGCCAAAC GCAACAGGAG
3541     GGGATACACT AGCAGCAGAC CGTTGCAAAC GCAGGACCTC CACTCCTCTT CTCCTCAACA
3601     CCCACTTTTG CCATCGAAAA ACCAGCCCAG TTATTGGGCT TGATTGGAGC TCGCTCATTC
3661     CAATTCCTTC TATTAGGCTA CTAACACCAT GACTTTATTA GCCTGTCTAT CCTGGCCCCC
3721     CTGGCGAGGT TCATGTTTGT TTATTTCCGA ATGCAACAAG CTCCGCATTA CACCCGAACA
3781     TCACTCCAGA TGAGGGCTTT CTGAGTGTGG GGTCAAATAG TTTCATGTTC CCCAAATGGC
3841     CCAAAACTGA CAGTTTAAAC GCTGTCTTGG AACCTAATAT GACAAAAGCG TGATCTCATC
3901     CAAGATGAAC TAAGTTTGGT TCGTTGAAAT GCTAACGGCC AGTTGGTCAA AAAGAAACTT
3961     CCAAAAGTCG GCATACCGTT TGTCTTGTTT GGTATTGATT GACGAATGCT CAAAAATAAT
4021     CTCATTAATG CTTAGCGCAG TCTCTCTATC GCTTCTGAAC CCCGGTGCAC CTGTGCCGAA
4081     ACGCAAATGG GGAAACACCC GCTTTTTGGA TGATTATGCA TTGTCTCCAC ATTGTATGCT
4141     TCCAAGATTC TGGTGGGAAT ACTGCTGATA GCCTAACGTT CATGATCAAA ATTTAACTGT
4201     TCTAACCCCT ACTTGACAGC AATATATAAA CAGAAGGAAG CTGCCCTGTC TTAAACCTTT
4261     TTTTTTATCA TCATTATTAG CTTACTTTCA TAATTGCGAC TGGTTCCAAT TGACAAGCTT
4321     TTGATTTTAA CGACTTTTAA CGACAACTTG AGAAGATCAA AAAACAACTA ATTATTCGAA
4381     GGATCCAAAC GATGAGATTT CCTTCAATTT TTACTGCAGT TTTATTCGCA GCATCCTCCG
4441     CATTAGCTGC TCCAGTCAAC ACTACAACAG AAGATGAAAC GGCACAAATT CCGGCTGAAG
4501     CTGTCATCGG TTACTCAGAT TTAGAAGGGG ATTTCGATGT TGCTGTTTTG CCATTTTCCA
4561     ACAGCACAAA TAACGGGTTA TTGTTTATAA ATACTACTAT TGCCAGCATT GCTGCTAAAG
4621     AAGAAGGGGT ATCTCTCGAG AAAAGAGAGG CTGAAGCTTA CGTAGAATTC CCTAGGGCGG
4681     CCGCGAATTA ATTCGCCTTA GACATGACTG TTCCTCAGTT CAAGTTGGGC ACTTACGAGA
4741     AGACCGGTCT TGCTAGATTC TAATCAAGAG GATGTCAGAA TGCCATTTGC CTGAGAGATG
4801     CAGGCTTCAT TTTTGATACT TTTTTATTTG TAACCTATAT AGTATAGGAT TTTTTTTGTC
4861     ATTTTGTTTC TTCTCGTACG AGCTTGCTCC TGATCAGCCT ATCTCGCAGC TGATGAATAT
4921     CTTGTGGTAG GGGTTTGGGA AAATCATTCG AGTTTGATGT TTTTCTTGGT ATTTCCCACT
4981     CCTCTTCAGA GTACAGAAGA TTAAGTGAGA CGTTCGTTTG TGCAAGCTTA TCGATAAGCT
5041     TTAATGCGGT AGTTTATCAC AGTTAAATTG CTAACGCAGT CAGGCACCGT GTATGAAATC
5101     TAACAATGCG CTCATCGTCA TCCTCGGCAC CGTCACCCTG GATGCTGTAG GCATAGGCTT
5161     GGTTATGCCG GTACTGCCGG GCCTCTTGCG GGATATCGTC CATTCCGACA GCATCGCCAG
5221     TCACTATGGC GTGCTGCTAG CGCTATATGC GTTGATGCAA TTTCTATGCG CACCCGTTCT
5281     CGGAGCACTG TCCGACCGCT TTGGCCGCCG CCCAGTCCTG CTCGCTTCGC TACTTGGAGC
5341     CACTATCGAC TACGCGATCA TGGCGACCAC ACCCGTCCTG TGGATCTATC GAATCTAAAT
5401     GTAAGTTAAA ATCTCTAAAT AATTAAATAA GTCCCAGTTT CTCCATACGA ACCTTAACAG
5461     CATTGCGGTG AGCATCTAGA CCTTCAACAG CAGCCAGATC CATCACTGCT TGGCCAATAT
5521     GTTTCAGTCC CTCAGGAGTT ACGTCTTGTG AAGTGATGAA CTTCTGGAAG GTTGCAGTGT
5581     TAACTCCGCT GTATTGACGG GCATATCCGT ACGTTGGCAA AGTGTGGTTG GTACCGGAGG
5641     AGTAATCTCC ACAACTCTCT GGAGAGTAGG CACCAACAAA CACAGATCCA GCGTGTTGTA
5701     CTTGATCAAC ATAAGAAGAA GCATTCTCGA TTTGCAGGAT CAAGTGTTCA GGAGCGTACT
5761     GATTGGACAT TTCCAAAGCC TGCTCGTAGG TTGCAACCGA TAGGGTTGTA GAGTGTGCAA
5821     TACACTTGCG TACAATTTCA ACCCTTGGCA ACTGCACAGC TTGGTTGTGA ACAGCATCTT
5881     CAATTCTGGC AAGCTCCTTG TCTGTCATAT CGACAGCCAA CAGAATCACC TGGGAATCAA
5941     TACCATGTTC AGCTTGAGAC AGAAGGTCTG AGGCAACGAA ATCTGGATCA GCGTATTTAT
6001     CAGCAATAAC TAGAACTTCA GAAGGCCCAG CAGGCATGTC AATACTACAC AGGGCTGATG
6061     TGTCATTTTG AACCATCATC TTGGCAGCAG TAACGAACTG GTTTCCTGGA CCAAATATTT
6121     TGTCACACTT AGGAACAGTT TCTGTTCCGT AAGCCATAGC AGCTACTGCC TGGGCGCCTC
6181     CTGCTAGCAC GATACACTTA GCACCAACCT TGTGGGCAAC GTAGATGACT TCTGGGGTAA
6241     GGGTACCATC CTTCTTAGGT GGAGATGCAA AAACAATTTC TTTGCAACCA GCAACTTTGG
6301     CAGGAACACC CAGCATCAGG GAAGTGGAAG GCAGAATTGC GGTTCCACCA GGAATATAGA
6361     GGCCAACTTT CTCAATAGGT CTTGCAAAAC GAGAGCAGAC TACACCAGGG CAAGTCTCAA
6421     CTTGCAACGT CTCCGTTAGT TGAGCTTCAT GGAATTTCCT GACGTTATCT ATAGAGAGAT
6481     CAATGGCTCT CTTAACGTTA TCTGGCAATT GCATAAGTTC CTCTGGGAAA GGAGCTTCTA
6541     ACACAGGTGT CTTCAAAGCG ACTCCATCAA ACTTGGCAGT TAGTTCTAAA AGGGCTTTGT
6601     CACCATTTTG ACGAACATTG TCGACAATTG GTTTGACTAA TTCCATAATC TGTTCCGTTT
6661     TCTGGATAGG ACGACGAAGG GCATCTTCAA TTTCTTGTGA GGAGGCCTTA GAAACGTCAA
6721     TTTTGCACAA TTCAATACGA CCTTCAGAAG GGACTTCTTT AGGTTTGGAT TCTTCTTTAG
6781     GTTGTTCCTT GGTGTATCCT GGCTTGGCAT CTCCTTTCCT TCTAGTGACC TTTAGGGACT
6841     TCATATCCAG GTTTCTCTCC ACCTCGTCCA ACGTCACACC GTACTTGGCA CATCTAACTA
6901     ATGCAAAATA AAATAAGTCA GCACATTCCC AGGCTATATC TTCCTTGGAT TTAGCTTCTG
6961     CAAGTTCATC AGCTTCCTCC CTAATTTTAG CGTTCAACAA AACTTCGTCG TCAAATAACC
7021     GTTTGGTATA AGAACCTTCT GGAGCATTGC TCTTACGATC CCACAAGGTG GCTTCCATGG
7081     CTCTAAGACC CTTTGATTGG CCAAAACAGG AAGTGCGTTC CAAGTGACAG AAACCAACAC
7141     CTGTTTGTTC AACCACAAAT TTCAAGCAGT CTCCATCACA ATCCAATTCG ATACCCAGCA
7201     ACTTTTGAGT TGCTCCAGAT GTAGCACCTT TATACCACAA ACCGTGACGA CGAGATTGGT
7261     AGACTCCAGT TTGTGTCCTT ATAGCCTCCG GAATAGACTT TTTGGACGAG TACACCAGGC
7321     CCAACGAGTA ATTAGAAGAG TCAGCCACCA AAGTAGTGAA TAGACCATCG GGGCGGTCAG
7381     TAGTCAAAGA CGCCAACAAA ATTTCACTGA CAGGGAACTT TTTGACATCT TCAGAAAGTT
7441     CGTATTCAGT AGTCAATTGC CGAGCATCAA TAATGGGGAT TATACCAGAA GCAACAGTGG
7501     AAGTCACATC TACCAACTTT GCGGTCTCAG AAAAAGCATA AACAGTTCTA CTACCGCCAT
7561     TAGTGAAACT TTTCAAATCG CCCAGTGGAG AAGAAAAAGG CACAGCGATA CTAGCATTAG
7621     CGGGCAAGGA TGCAACTTTA TCAACCAGGG TCCTATAGAT AACCCTAGCG CCTGGGATCA
7681     TCCTTTGGAC AACTCTTTCT GCCAAATCTA GGTCCAAAAT CACTTCATTG ATACCATTAT
7741     TGTACAACTT GAGCAAGTTG TCGATCAGCT CCTCAAATTG GTCCTCTGTA ACGGATGACT
7801     CAACTTGCAC ATTAACTTGA AGCTCAGTCG ATTGAGTGAA CTTGATCAGG TTGTGCAGCT
7861     GGTCAGCAGC ATAGGGAAAC ACGGCTTTTC CTACCAAACT CAAGGAATTA TCAAACTCTG
7921     CAACACTTGC GTATGCAGGT AGCAAGGGAA ATGTCATACT TGAAGTCGGA CAGTGAGTGT
7981     AGTCTTGAGA AATTCTGAAG CCGTATTTTT ATTATCAGTG AGTCAGTCAT CAGGAGATCC
8041     TCTACGCCGG ACGCATCGTG GCCGACCTGC AGGGGGGGGG GGCGCTGAGG TCTGCCTCGT
8101     GAAGAAGGTG TTGCTGACTC ATACCAGGCC TGAATCGCCC CATCATCCAG CCAGAAAGTG
8161     AGGGAGCCAC GGTTGATGAG AGCTTTGTTG TAGGTGGACC AGTTGGTGAT TTTGAACTTT
8221     TGCTTTGCCA CGGAACGGTC TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA
8281     GCAAAAGTTC GATTTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA ATGCTCTGCC
8341     AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC AAATGAAACT
8401     GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG
8461     AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA
8521     TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT
8581     CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGCTTATGCA
8641     TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT
8701     CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT
8761     TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT
8821     CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG
8881     GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG
8941     GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG
9001     CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC
9061     GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT
9121     CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT TGAATATGGC
9181     TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA
9241     TATTTTTATC TTGTGCAATG TAACAT
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