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Human immunodeficiency virus type 2, isolate ROD, complete proviral genome

发布时间：2026/4/13 8:16:40 阅读次数：4

Human immunodeficiency virus type 2, isolate ROD, complete proviral genome
GenBank: M15390.1
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LOCUS       HIV2ROD                 9671 bp    DNA     linear   VRL 14-JUL-2016
DEFINITION Human immunodeficiency virus type 2, isolate ROD, complete proviral
            genome.
ACCESSION   M15390
VERSION     M15390.1
KEYWORDS    TAR protein; acquired immune deficiency syndrome; complete genome;
            env protein; gag protein; long terminal repeat; pol protein;
            polyprotein; reverse transcriptase; transactivator.
SOURCE      Human immunodeficiency virus 2 (HIV-2)
ORGANISM Human immunodeficiency virus 2
            Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
            Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
REFERENCE   1 (bases 9361 to 9671)
AUTHORS   Clavel,F., Guyader,M., Guetard,D., Salle,M., Montagnier,L. and
            Alizon,M.
TITLE     Molecular cloning and polymorphism of the human immune deficiency
            virus type 2
JOURNAL   Nature 324 (6098), 691-695 (1986)
   PUBMED   3025743
REFERENCE   2 (bases 1 to 9671)
AUTHORS   Guyader,M., Emerman,M., Sonigo,P., Clavel,F., Montagnier,L. and
            Alizon,M.
TITLE     Genome organization and transactivation of the human
            immunodeficiency virus type 2
JOURNAL   Nature 326 (6114), 662-669 (1987)
   PUBMED   3031510
COMMENT     On May 24, 1996 this sequence version replaced gi:325742.
            Original source text: Human immunodeficiency virus 2
            (individual_isolate ROD) (clone: lambda ROD[27,35].) DNA.
            The ROD isolate was obtained from offshore Senegal in 1985 and this
            seqence was subsequently determined from two clones: positions 1 to
            2658 from ROD 27 and positions 2659 to 9671 from ROD 35. The X ORF
            has not been found in HIV1. Because the authors claim 100-fold
            cross-transactivation, we have tentatively annotated the tat
            protein as tat-III, although the HIV2-tat is somewhat larger and
            may exhibit a differing specificity.
            An infectious clone of this virus has been constructed by Keith
            Peden, Molecular Biology and Genetics, Johns Hopkins University
            School of Medicine, Baltimore, MD. 21205 (301)955-3652.
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Human immunodeficiency virus 2"
                     /proviral
                     /mol_type="genomic DNA"
                     /isolate="ROD"
                     /db_xref="taxon:11709"
                     /clone="lambda ROD[27,35]."
     repeat_region   <1..299
                     /note="5' LTR"
                     /rpt_type=long_terminal_repeat
     repeat_region   <1..173
                     /note="R repeat 5' copy"
     misc_binding    303..319
                     /bound_moiety="Lys-tRNA primer"
     CDS             546..2114
                     /note="gag polyprotein"
                     /codon_start=1
                     /protein_id="AAB00763.1"
                     /translation="MGARNSVLRGKKADELERIRLRPGGKKKYRLKHIVWAANKLDRF
                     GLAESLLESKEGCQKILTVLDPMVPTGSENLKSLFNTVCVIWCIHAEEKVKDTEGAKQ
                     IVRRHLVAETGTAEKMPSTSRPTAPSSEKGGNYPVQHVGGNYTHIPLSPRTLNAWVKL
                     VEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIREIINEEAAEWDVQH
                     PIPGPLPAGQLREPRGSDIAGTTSTVEEQIQWMFRPQNPVPVGNIYRRWIQIGLQKCV
                     RMYNPTNILDIKQGPKEPFQSYVDRFYKSLRAEQTDPAVKNWMTQTLLVQNANPDCKL
                     VLKGLGMNPTLEEMLTACQGVGGPGQKARLMAEALKEVIGPAPIPFAAAQQRKAFKCW
                     NCGKEGHSARQCRAPRRQGCWKCGKPGHIMTNCPDRQAGFLGLGPWGKKPRNFPVAQV
                     PQGLTPTAPPVDPAVDLLEKYMQQGKRQREQRERPYKEVTEDLLHLEQGETPYREPPT
                     EDLLHLNSLFGKDQ"
     gene            2309..4939
                     /gene="pol"
     CDS             2309..4939
                     /gene="pol"
                     /note="ORF pol polypeptide bordered by stop codons
                     (1829-4939) first start codon at 2309"
                     /codon_start=1
                     /product="pol polyprotein"
                     /protein_id="AAB00764.1"
                     /translation="MTGDTPINIFGRNILTALGMSLNLPVAKVEPIKIMLKPGKDGPK
                     LRQWPLTKEKIEALKEICEKMEKEGQLEEAPPTNPYNTPTFAIKKKDKNKWRMLIDFR
                     ELNKVTQDFTEIQLGIPHPAGLAKKRRITVLDVGDAYFSIPLHEDFRPYTAFTLPSVN
                     NAEPGKRYIYKVLPQGWKGSPAIFQHTMRQVLEPFRKANKDVIIIQYMDDILIASDRT
                     DLEHDRVVLQLKELLNGLGFSTPDEKFQKDPPYHWMGYELWPTKWKLQKIQLPQKEIW
                     TVNDIQKLVGVLNWAAQLYPGIKTKHLCRLIRGKMTLTEEVQWTELAEAELEENRIIL
                     SQEQEGHYYQEEKELEATVQKDQENQWTYKIHQEEKILKVGKYAKVKNTHTNGIRLLA
                     QVVQKIGKEALVIWGRIPKFHLPVEREIWEQWWDNYWQVTWIPDWDFVSTPPLVRLAF
                     NLVGDPIPGAETFYTDGSCNRQSKEGKAGYVTDRGKDKVKKLEQTTNQQAELEAFAMA
                     LTDSGPKVNIIVDSQYVMGISASQPTESESKIVNQIIEEMIKKEAIYVAWVPAHKGIG
                     GNQEVDHLVSQGIRQVLFLEKIEPAQEEHEKYHSNVKELSHKFGIPNLVARQIVNSCA
                     QCQQKGEAIHGQVNAELGTWQMDCTHLEGKIIIVAVHVASGFIEAEVIPQESGRQTAL
                     FLLKLASRWPITHLHTDNGANFTSQEVKMVAWWIGIEQSFGVPYNPQSQGVVEAMNHH
                     LKNQISRIREQANTIETIVLMAIHCMNFKRRGGIGDMTPSERLINMITTEQEIQFLQA
                     KNSKLKDFRVYFREGRDQLWKGPGELLWKGEGAVLVKVGTDIKIIPRRKAKIIRDYGG
                     RQEMDSGSHLEGAREDGEMA"
     CDS             4869..5516
                     /note="vif protein"
                     /codon_start=1
                     /protein_id="AAB00765.1"
                     /translation="MEEDKRWIVVPTWRVPGRMEKWHSLVKYLKYKTKDLEKVCYVPH
                     HKVGWAWWTCSRVIFPLKGNSHLEIQAYWNLTPEKGWLSSYSVRITWYTEKFWTDVTP
                     DCADVLIHSTYFPCFTAGEVRRAIRGEKLLSCCNYPRAHRAQVPSLQFLALVVVQQND
                     RPQRDSTTRKQRRRDYRRGLRLAKQDSRSHKQRSSESPTPRTYFPGVAEVLEILA"
     CDS             5344..5682
                     /note="vpx protein"
                     /codon_start=1
                     /protein_id="AAB00766.1"
                     /translation="MTDPRETVPPGNSGEETIGEAFAWLNRTVEAINREAVNHLPREL
                     IFQVWQRSWRYWHDEQGMSESYTKYRYLCIIQKAVYMHVRKGCTCLGRGHGPGGWRPG
                     PPPPPPPGLV"
     CDS             5682..5999
                     /note="vpr protein"
                     /codon_start=1
                     /protein_id="AAB00767.1"
                     /translation="MAEAPTELPPVDGTPLREPGDEWIIEILREIKEEALKHFDPRLL
                     IALGKYIYTRHGDTLEGARELIKVLQRALFTHFRAGCGHSRIGQTRGGNPLSAIPTPR
                     NMQ"
     CDS             join(5845..6140,8307..8403)
                     /note="tat protein"
                     /codon_start=1
                     /protein_id="AAB00768.1"
                     /translation="METPLKAPESSLKSCNEPFSRTSEQDVATQELARQGEEILSQLY
                     RPLETCNNSCYCKRCCYHCQMCFLNKGLGICYERKGRRRRTPKKTKTHPSPTPDKSIS
                     TRTGDSQPTKKQKKTVEATVETDTGPGR"
     exon            <5845..6140
                     /note="tat protein, (first expressed exon)"
                     /number=2
     CDS             join(6071..6140,8307..8539)
                     /note="rev protein"
                     /codon_start=1
                     /protein_id="AAB00769.1"
                     /translation="MNERADEEGLQRKLRLIRLLHQTNPYPQGPGTASQRRNRRRRWK
                     QRWRQILALADSIYTFPDPPADSPLDQTIQHLQGLTIQELPDPPTHLPESQRLAET"
     exon            <6071..6140
                     /note="rev protein, (first expressed exon)"
                     /number=2
     intron          6141..8306
                     /note="rev cds intron 2"
     intron          6141..8306
                     /note="tat cds intron 2"
     intron          6141..8306
                     /note="tat, rev, nef subgenomic mRNA intron 2"
     CDS             6147..8723
                     /note="env polyprotein"
                     /codon_start=1
                     /protein_id="AAB00770.1"
                     /translation="MMNQLLIAILLASACLVYCTQYVTVFYGVPTWKNATIPLFCATR
                     NRDTWGTIQCLPDNDDYQEITLNVTEAFDAWNNTVTEQAIEDVWHLFETSIKPCVKLT
                     PLCVAMKCSSTESSTGNNTTSKSTSTTTTTPTDQEQEISEDTPCARADNCSGLGEEET
                     INCQFNMTGLERDKKKQYNETWYSKDVVCETNNSTNQTQCYMNHCNTSVITESCDKHY
                     WDAIRFRYCAPPGYALLRCNDTNYSGFAPNCSKVVASTCTRMMETQTSTWFGFNGTRA
                     ENRTYIYWHGRDNRTIISLNKYYNLSLHCKRPGNKIVKQIMLMSGHVFHSHYQPINKR
                     PRQAWCWFKGKWKDAMQEVKETLAKHPRYRGTNDTRNISFAAPGKGSDPEVAYMWTNC
                     RGEFLYCNMTWFLNWIENKTHRNYAPCHIKQIINTWHKVGRNVYLPPREGELSCNSTV
                     TSIIANIDWQNNNQTNITFSAEVAELYRLELGDYKLVEITPIGFAPTKEKRYSSAHGR
                     HTRGVFVLGFLGFLATAGSAMGAASLTVSAQSRTLLAGIVQQQQQLLDVVKRQQELLR
                     LTVWGTKNLQARVTAIEKYLQDQARLNSWGCAFRQVCHTTVPWVNDSLAPDWDNMTWQ
                     EWEKQVRYLEANISKSLEQAQIQQEKNMYELQKLNSWDIFGNWFDLTSWVKYIQYGVL
                     IIVAVIALRIVIYVVQMLSRLRKGYRPVFSSPPGYIQQIHIHKDRGQPANEETEEDGG
                     SNGGDRYWPWPIAYIHFLIRQLIRLLTRLYSICRDLLSRSFLTLQLIYQNLRDWLRLR
                     TAFLQYGCEWIQEAFQAAARATRETLAGACRGLWRVLERIGRGILAVPRRIRQGAEIA
                     LL"
     exon            8307..>8539
                     /note="rev protein"
                     /number=3
     exon            8307..>8403
                     /note="tat protein, (AA at 8308)"
                     /number=3
     CDS             8557..9327
                     /note="nef protein"
                     /codon_start=1
                     /protein_id="AAB00771.1"
                     /translation="MGASGSKKHSRPPRGLQERLLRARAGACGGYWNESGGEYSRFQE
                     GSDREQKSPSCEGRQYQQGDFMNTPWKDPAAEREKNLYRQQNMDDVDSDDDDQVRVSV
                     TPKVPLRPMTHRLAIDMSHLIKTRGGLEGMFYSERRHKILNIYLEKEEGIIADWQNYT
                     HGPGVRYPMFFGWLWKLVPVDVPQEGEDTETHCLVHPAQTSKFDDPHGETLVWEFDPL
                     LAYSYEAFIRYPEEFGHKSGLPEEEWKARLKARGIPFS"
     repeat_region   8942..>9671
                     /note="3' LTR"
                     /rpt_type=long_terminal_repeat
     repeat_region   9497..9671
                     /note="R repeat 3' copy"
     regulatory      9649..9654
                     /regulatory_class="polyA_signal_sequence"
                     /note="mRNA polyadenylation signal"
ORIGIN      Cap site of genomic RNA.
        1 ggtcgctctg cggagaggct ggcagattga gccctgggag gttctctcca gcactagcag
       61 gtagagcctg ggtgttccct gctagactct caccagcact tggccggtgc tgggcagacg
      121 gccccacgct tgcttgctta aaaacctctt aataaagctg ccagttagaa gcaagttaag
      181 tgtgtgctcc catctctcct agtcgccgcc tggtcattcg gtgttcacct gagtaacaag
      241 accctggtct gttaggaccc ttcttgcttt gggaaaccga ggcaggaaaa tccctagcag
      301 gttggcgcct gaacagggac ttgaagaaga ctgagaagtc ttggaacacg gctgagtgaa
      361 ggcagtaagg gcggcaggaa caaaccacga cggagtgctc ctagaaaggc gcgggccgag
      421 gtaccaaagg cagcgtgtgg agcgggagga gaagaggcct ccgggtgaag gtaagtacct
      481 acaccaaaaa ctgtagccga aagggcttgc tatcctacct ttagacaggt agaagattgt
      541 gggagatggg cgcgagaaac tccgtcttga gagggaaaaa agcagatgaa ttagaaagaa
      601 tcaggttacg gcccggcgga aagaaaaagt acaggctaaa acatattgtg tgggcagcga
      661 ataaattgga cagattcgga ttagcagaga gcctgttgga gtcaaaagag ggttgtcaaa
      721 aaattcttac agttttagat ccaatggtac cgacaggttc agaaaattta aaaagtcttt
      781 ttaatactgt ctgcgtcatt tggtgcatac acgcagaaga gaaagtgaaa gatactgaag
      841 gagcaaaaca aatagtgcgg agacatctag tggcagaaac aggaactgca gagaaaatgc
      901 caagcacaag tagaccaaca gcaccatcta gcgagaaggg aggaaattac ccagtgcaac
      961 atgtaggcgg caactacacc catataccgc tgagtccccg aaccctaaat gcctgggtaa
     1021 aattagtaga ggaaaaaaag ttcggggcag aagtagtgcc aggatttcag gcactctcag
     1081 aaggctgcac gccctatgat atcaaccaaa tgcttaattg tgtgggcgac catcaagcag
     1141 ccatgcagat aatcagggag attatcaatg aggaagcagc agaatgggat gtgcaacatc
     1201 caataccagg ccccttacca gcggggcagc ttagagagcc aaggggatct gacatagcag
     1261 ggacaacaag cacagtagaa gaacagatcc agtggatgtt taggccacaa aatcctgtac
     1321 cagtaggaaa catctataga agatggatcc agataggatt gcagaagtgt gtcaggatgt
     1381 acaacccgac caacatccta gacataaaac agggaccaaa ggagccgttc caaagctatg
     1441 tagatagatt ctacaaaagc ttgagggcag aacaaacaga tccagcagtg aagaattgga
     1501 tgacccaaac actgctagta caaaatgcca acccagactg taaattagtg ctaaaaggac
     1561 tagggatgaa ccctacctta gaagagatgc tgaccgcctg tcagggggta ggtgggccag
     1621 gccagaaagc tagattaatg gcagaggccc tgaaagaggt cataggacct gcccctatcc
     1681 cattcgcagc agcccagcag agaaaggcat ttaaatgctg gaactgtgga aaggaagggc
     1741 actcggcaag acaatgccga gcacctagaa ggcagggctg ctggaagtgt ggtaagccag
     1801 gacacatcat gacaaactgc ccagatagac aggcaggttt tttaggactg ggcccttggg
     1861 gaaagaagcc ccgcaacttc cccgtggccc aagttccgca ggggctgaca ccaacagcac
     1921 ccccagtgga tccagcagtg gatctactgg agaaatatat gcagcaaggg aaaagacaga
     1981 gagagcagag agagagacca tacaaggaag tgacagagga cttactgcac ctcgagcagg
     2041 gggagacacc atacagggag ccaccaacag aggacttgct gcacctcaat tctctctttg
     2101 gaaaagacca gtagtcacag catacattga gggtcagcca gtagaagtct tgttagacac
     2161 aggggctgac gactcaatag tagcaggaat agagttaggg aacaattata gcccaaaaat
     2221 agtaggggga atagggggat tcataaatac caaggaatat aaaaatgtag aaatagaagt
     2281 tctaaataaa aaggtacggg ccaccataat gacaggcgac accccaatca acatttttgg
     2341 cagaaatatt ctgacagcct taggcatgtc attaaatcta ccagtcgcca aagtagagcc
     2401 aataaaaata atgctaaagc cagggaaaga tggaccaaaa ctgagacaat ggcccttaac
     2461 aaaagaaaaa atagaagcac taaaagaaat ctgtgaaaaa atggaaaaag aaggccagct
     2521 agaggaagca cctccaacta atccttataa tacccccaca tttgcaatca agaaaaagga
     2581 caaaaacaaa tggaggatgc taatagattt cagagaacta aacaaggtaa ctcaagattt
     2641 cacagaaatt cagttaggaa ttccacaccc agcagggttg gccaagaaga gaagaattac
     2701 tgtactagat gtaggggatg cttacttttc cataccacta catgaggact ttagaccata
     2761 tactgcattt actctaccat cagtgaacaa tgcagaacca ggaaaaagat acatatataa
     2821 agtcttgcca cagggatgga agggatcacc agcaattttt caacacacaa tgagacaggt
     2881 attagaacca ttcagaaaag caaacaagga tgtcattatc attcagtaca tggatgatat
     2941 cttaatagct agtgacagga cagatttaga acatgatagg gtagtcctgc agctcaagga
     3001 acttctaaat ggcctaggat tttctacccc agatgagaag ttccaaaaag accctccata
     3061 ccactggatg ggctatgaac tatggccaac taaatggaag ttgcagaaaa tacagttgcc
     3121 ccaaaaagaa atatggacag tcaatgacat ccagaagcta gtgggtgtcc taaattgggc
     3181 agcacaactc tacccaggga taaagaccaa acacttatgt aggttaatca gaggaaaaat
     3241 gacactcaca gaagaagtac agtggacaga attagcagaa gcagagctag aagaaaacag
     3301 aattatccta agccaggaac aagagggaca ctattaccaa gaagaaaaag agctagaagc
     3361 aacagtccaa aaggatcaag agaatcagtg gacatataaa atacaccagg aagaaaaaat
     3421 tctaaaagta ggaaaatatg caaaggtgaa aaacacccat accaatggaa tcagattgtt
     3481 agcacaggta gttcagaaaa taggaaaaga agcactagtc atttggggac gaataccaaa
     3541 atttcaccta ccagtagaga gagaaatctg ggagcagtgg tgggataact actggcaagt
     3601 gacatggatc ccagactggg acttcgtgtc taccccacca ctggtcaggt tagcgtttaa
     3661 cctggtaggg gatcctatac caggtgcaga gaccttctac acagatggat cctgcaatag
     3721 gcaatcaaaa gaaggaaaag caggatatgt aacagataga gggaaagaca aggtaaagaa
     3781 actagagcaa actaccaatc agcaagcaga actagaagcc tttgcgatgg cactaacaga
     3841 ctcgggtcca aaagttaata ttatagtaga ctcacagtat gtaatgggga tcagtgcaag
     3901 ccaaccaaca gagtcagaaa gtaaaatagt gaaccagatc atagaagaaa tgataaaaaa
     3961 ggaagcaatc tatgttgcat gggtcccagc ccacaaaggc atagggggaa accaggaagt
     4021 agatcattta gtgagtcagg gtatcagaca agtgttgttc ctggaaaaaa tagagcccgc
     4081 tcaggaagaa catgaaaaat atcatagcaa tgtaaaagaa ctgtctcata aatttggaat
     4141 acccaattta gtggcaaggc aaatagtaaa ctcatgtgcc caatgtcaac agaaagggga
     4201 agctatacat gggcaagtaa atgcagaact aggcacttgg caaatggact gcacacattt
     4261 agaaggaaag atcattatag tagcagtaca tgttgcaagt ggatttatag aagcagaagt
     4321 catcccacag gaatcaggaa gacaaacagc actcttccta ttgaaactgg caagtaggtg
     4381 gccaataaca cacttgcata cagataatgg tgccaacttc acttcacagg aggtgaagat
     4441 ggtagcatgg tggataggta tagaacaatc ctttggagta ccttacaatc cacagagcca
     4501 aggagtagta gaagcaatga atcaccatct aaaaaaccaa ataagtagaa tcagagaaca
     4561 ggcaaataca atagaaacaa tagtactaat ggcaattcat tgcatgaatt ttaaaagaag
     4621 ggggggaata ggggatatga ctccatcaga aagattaatc aatatgatca ccacagaaca
     4681 agagatacaa ttcctccaag ccaaaaattc aaaattaaaa gattttcggg tctatttcag
     4741 agaaggcaga gatcagttgt ggaaaggacc tggggaacta ctgtggaaag gagaaggagc
     4801 agtcctagtc aaggtaggaa cagacataaa aataatacca agaaggaaag ccaagatcat
     4861 cagagactat ggaggaagac aagagatgga tagtggttcc cacctggagg gtgccaggga
     4921 ggatggagaa atggcatagc cttgtcaagt atctaaaata caaaacaaag gatctagaaa
     4981 aggtgtgcta tgttccccac cataaggtgg gatgggcatg gtggacttgc agcagggtaa
     5041 tattcccatt aaaaggaaac agtcatctag agatacaggc atattggaac ttaacaccag
     5101 aaaaaggatg gctctcctct tattcagtaa gaataacttg gtacacagaa aagttctgga
     5161 cagatgttac cccagactgt gcagatgtcc taatacatag cacttatttc ccttgcttta
     5221 cagcaggtga agtaagaaga gccatcagag gggaaaagtt attgtcctgc tgcaattatc
     5281 cccgagctca tagagcccag gtaccgtcac ttcaatttct ggccttagtg gtagtgcaac
     5341 aaaatgacag accccagaga gacagtacca ccaggaaaca gcggcgaaga gactatcgga
     5401 gaggccttcg cctggctaaa caggacagta gaagccataa acagagaagc agtgaatcac
     5461 ctaccccgag aacttatttt ccaggtgtgg cagaggtcct ggagatactg gcatgatgaa
     5521 caagggatgt cagaaagtta cacaaagtat agatatttgt gcataataca gaaagcagtg
     5581 tacatgcatg ttaggaaagg gtgtacttgc ctggggaggg gacatgggcc aggagggtgg
     5641 agaccagggc ctcctcctcc tccccctcca ggtctggtct aatggctgaa gcaccaacag
     5701 agctcccccc ggtggatggg accccactga gggagccagg ggatgagtgg ataatagaaa
     5761 tcttgagaga aataaaagaa gaagctttaa agcattttga ccctcgcttg ctaattgctc
     5821 ttggcaaata tatctatact agacatggag acacccttga aggcgccaga gagctcatta
     5881 aagtcctgca acgagccctt ttcacgcact tcagagcagg atgtggccac tcaagaattg
     5941 gccagacaag gggaggaaat cctctctcag ctataccgac ccctagaaac atgcaataac
     6001 tcatgctatt gtaagcgatg ctgctaccat tgtcagatgt gttttctaaa caaggggctc
     6061 gggatatgtt atgaacgaaa gggcagacga agaaggactc caaagaaaac taagactcat
     6121 ccgtctccta caccagacaa gtgagtatga tgaatcagct gcttattgcc attttattag
     6181 ctagtgcttg cttagtatat tgcacccaat atgtaactgt tttctatggc gtacccacgt
     6241 ggaaaaatgc aaccattccc ctcttttgtg caaccagaaa tagggatact tggggaacca
     6301 tacagtgctt gcctgacaat gatgattatc aggaaataac tttgaatgta acagaggctt
     6361 ttgatgcatg gaataataca gtaacagaac aagcaataga agatgtctgg catctattcg
     6421 agacatcaat aaaaccatgt gtcaaactaa cacctttatg tgtagcaatg aaatgcagca
     6481 gcacagagag cagcacaggg aacaacacaa cctcaaagag cacaagcaca accacaacca
     6541 cacccacaga ccaggagcaa gagataagtg aggatactcc atgcgcacgc gcagacaact
     6601 gctcaggatt gggagaggaa gaaacgatca attgccagtt caatatgaca ggattagaaa
     6661 gagataagaa aaaacagtat aatgaaacat ggtactcaaa agatgtggtt tgtgagacaa
     6721 ataatagcac aaatcagacc cagtgttaca tgaaccattg caacacatca gtcatcacag
     6781 aatcatgtga caagcactat tgggatgcta taaggtttag atactgtgca ccaccgggtt
     6841 atgccctatt aagatgtaat gataccaatt attcaggctt tgcacccaac tgttctaaag
     6901 tagtagcttc tacatgcacc aggatgatgg aaacgcaaac ttccacatgg tttggcttta
     6961 atggcactag agcagagaat agaacatata tctattggca tggcagagat aatagaacta
     7021 tcatcagctt aaacaaatat tataatctca gtttgcattg taagaggcca gggaataaga
     7081 tagtgaaaca aataatgctt atgtcaggac atgtgtttca ctcccactac cagccgatca
     7141 ataaaagacc cagacaagca tggtgctggt tcaaaggcaa atggaaagac gccatgcagg
     7201 aggtgaagga aacccttgca aaacatccca ggtatagagg aaccaatgac acaaggaata
     7261 ttagctttgc agcgccagga aaaggctcag acccagaagt agcatacatg tggactaact
     7321 gcagaggaga gtttctctac tgcaacatga cttggttcct caattggata gagaataaga
     7381 cacaccgcaa ttatgcaccg tgccatataa agcaaataat taacacatgg cataaggtag
     7441 ggagaaatgt atatttgcct cccagggaag gggagctgtc ctgcaactca acagtaacca
     7501 gcataattgc taacattgac tggcaaaaca ataatcagac aaacattacc tttagtgcag
     7561 aggtggcaga actatacaga ttggagttgg gagattataa attggtagaa ataacaccaa
     7621 ttggcttcgc acctacaaaa gaaaaaagat actcctctgc tcacgggaga catacaagag
     7681 gtgtgttcgt gctagggttc ttgggttttc tcgcaacagc aggttctgca atgggcgcgg
     7741 cgtccctgac cgtgtcggct cagtcccgga ctttactggc cgggatagtg cagcaacagc
     7801 aacagctgtt ggacgtggtc aagagacaac aagaactgtt gcgactgacc gtctggggaa
     7861 cgaaaaacct ccaggcaaga gtcactgcta tagagaagta cctacaggac caggcgcggc
     7921 taaattcatg gggatgtgcg tttagacaag tctgccacac tactgtacca tgggttaatg
     7981 attccttagc acctgactgg gacaatatga cgtggcagga atgggaaaaa caagtccgct
     8041 acctggaggc aaatatcagt aaaagtttag aacaggcaca aattcagcaa gagaaaaata
     8101 tgtatgaact acaaaaatta aatagctggg atatttttgg caattggttt gacttaacct
     8161 cctgggtcaa gtatattcaa tatggagtgc ttataatagt agcagtaata gctttaagaa
     8221 tagtgatata tgtagtacaa atgttaagta ggcttagaaa gggctatagg cctgttttct
     8281 cttccccccc cggttatatc caacagatcc atatccacaa ggaccgggga cagccagcca
     8341 acgaagaaac agaagaagac ggtggaagca acggtggaga cagatactgg ccctggccga
     8401 tagcatatat acatttcctg atccgccagc tgattcgcct cttgaccaga ctatacagca
     8461 tctgcaggga cttactatcc aggagcttcc tgaccctcca actcatctac cagaatctca
     8521 gagactggct gagacttaga acagccttct tgcaatatgg gtgcgagtgg atccaagaag
     8581 cattccaggc cgccgcgagg gctacaagag agactcttgc gggcgcgtgc aggggcttgt
     8641 ggagggtatt ggaacgaatc gggaggggaa tactcgcggt tccaagaagg atcagacagg
     8701 gagcagaaat cgccctcctg tgagggacgg cagtatcagc agggagactt tatgaatact
     8761 ccatggaagg acccagcagc agaaagggag aaaaatttgt acaggcaaca aaatatggat
     8821 gatgtagatt cagatgatga tgaccaagta agagtttctg tcacaccaaa agtaccacta
     8881 agaccaatga cacatagatt ggcaatagat atgtcacatt taataaaaac aaggggggga
     8941 ctggaaggga tgttttacag tgaaagaaga cataaaatct taaatatata cttagaaaag
     9001 gaagaaggga taattgcaga ttggcagaac tacactcatg ggccaggagt aagataccca
     9061 atgttctttg ggtggctatg gaagctagta ccagtagatg tcccacaaga aggggaggac
     9121 actgagactc actgcttagt acatccagca caaacaagca agtttgatga cccgcatggg
     9181 gagacactag tctgggagtt tgatcccttg ctggcttata gttacgaggc ttttattcgg
     9241 tacccagagg aatttgggca caagtcaggc ctgccagagg aagagtggaa ggcgagactg
     9301 aaagcaagag gaataccatt tagttaaaga caggaacagc tatacttggt cagggcagga
     9361 agtaactaac agaaacagct gagactgcag ggactttcca gaaggggctg taaccaaggg
     9421 agggacatgg gaggagctgg tggggaacgc cctcatattc tctgtataaa tatacccgct
     9481 agcttgcatt gtacttcggt cgctctgcgg agaggctggc agattgagcc ctgggaggtt
     9541 ctctccagca gtagcaggta gagcctgggt gttccctgct agactctcac cagcacttgg
     9601 ccggtgctgg gcagacggcc ccacgcttgc ttgcttaaaa acctccttaa taaagctgcc
     9661 agttagaagc a
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