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Human respiratory syncytial virus B strain HRSV/B/Texas.USA/121301314/2014, complete genome

发布时间：2026/4/13 8:21:04 阅读次数：14

Human respiratory syncytial virus B strain HRSV/B/Texas.USA/121301314/2014, complete genome
GenBank: OK649755.1
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LOCUS       OK649755               15251 bp    cRNA    linear   VRL 07-NOV-2021
DEFINITION Human respiratory syncytial virus B strain
            HRSV/B/Texas.USA/121301314/2014, complete genome.
ACCESSION   OK649755
VERSION     OK649755.1
DBLINK      BioProject: PRJNA322324
KEYWORDS    .
SOURCE      Human respiratory syncytial virus B
ORGANISM Human respiratory syncytial virus B
            Viruses; Riboviria; Orthornavirae; Negarnaviricota;
            Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae;
            Orthopneumovirus.
REFERENCE   1 (bases 1 to 15251)
AUTHORS   Wang,L., Ng,T.F.F., Castro,C.J., Marine,R.L., Magana,L.C.,
            Esona,M., Peret,T.C.T. and Thornburg,N.J.
TITLE     Next-Generation Sequencing of Human Respiratory Syncytial Virus
            Subgroups A and B Genomes
JOURNAL   J Virol Methods (2021) In press
   PUBMED   34673119
REMARK    Publication Status: Available-Online prior to print
REFERENCE   2 (bases 1 to 15251)
AUTHORS   Wang,L., Ng,T.F.F., Castro,C.J., Marine,R.L., Magana,L.C.,
            Esona,M., Peret,T.C.T. and Thornburg,N.J.
TITLE     Direct Submission
JOURNAL   Submitted (22-OCT-2021) Respiratory Viruses Branch, Division of
            Viral Diseases, Centers for Disease Control and Prevention, 1600
            Clifton Rd. NE, Atlanta, GA 30329, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.9; Geneious v. R11
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Human respiratory syncytial virus B"
                     /mol_type="viral cRNA"
                     /strain="HRSV/B/Texas.USA/121301314/2014"
                     /host="Homo sapiens"
                     /db_xref="taxon:208895"
                     /country="USA"
                     /collection_date="2014"
                     /note="subtype: B"
     CDS             91..510
                     /note="NS1"
                     /codon_start=1
                     /product="nonstructural protein 1"
                     /protein_id="UDY80215.1"
                     /translation="MGCNSLSMIKVRLQNLFDNDEVALLKITCYTDKLILLTNALAKA
                     TIHTIKLNGIVFIHVITSSEVCPDNNIVVKSNFTTMPILQNGGYIWELIELTHCSQLN
                     GLIDDNCEIKFSKRLSDSVMTDYMNQISDLLGLDLHS"
     CDS             618..992
                     /note="NS2"
                     /codon_start=1
                     /product="nonstructural protein 2"
                     /protein_id="UDY80216.1"
                     /translation="MSTTNDNTTMQRLMITDMRPLSMDSIITSLTKEIITHKFIYLIN
                     NECIVRKLDERQATFTFLVNYEMKLLHKVGSTKYKKYTEYNTKYGTFPMPIFINHGGF
                     LECIGIKPTKHTPIIYKYDLNP"
     CDS             1131..2306
                     /note="N"
                     /codon_start=1
                     /product="nucleoprotein"
                     /protein_id="UDY80217.1"
                     /translation="MALSKVKLNDTLNKDQLLSSSKYTIQRSTGDNIDTPNYDVQKHL
                     NKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILKDAGYHVKANGVDITTYRQ
                     DINGKEMKFEVLTLSSLTSEIQVNIEIESRKSYKKMLKEMGEVAPEYRHDSPDCGMII
                     LCIAALVITKLAAGDRSGLTAVIRRANNVLKNEIKRYKGLIPKDIANSFYEVFEKHPH
                     LIDVFVHFGIAQSSTRGGSRVEGIFAGLFMNAYGSGQVMLRWGVLAKSVKNIMLGHAS
                     VQAEMEQVVEVYEYAQKLGGEAGFYHILNNPKASLLSLTQFPNFSSVVLGNAAGLGIM
                     GEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDLTTEELEAIKHQLNPKEDDVEL"
     CDS             2339..3064
                     /note="P"
                     /codon_start=1
                     /product="phosphoprotein"
                     /protein_id="UDY80218.1"
                     /translation="MEKFAPEFHGEDANNKATKFLESIKGKFASSKDPKKKDSIISVN
                     SIDIEVTKESPITSGTNIINPTSEADSTLETKANYPRKPLVSFKEDLTPSDNPFSKLY
                     KETIETFDNNEEESSYSYEEINDQTNDNITARLDRIDEKLSEILGMLHTLVVASAGPT
                     SARDGIRDAMVGLREEMIEKIRAEALMTNDRLEAMARLRNEESEKMTKDTSDEVSLNP
                     TSKKLSDLLEDNDSDNDLSLDDF"
     CDS             3255..4025
                     /note="M"
                     /codon_start=1
                     /product="matrix protein"
                     /protein_id="UDY80219.1"
                     /translation="METYVNKLHEGSTYTAAVQYNVLEKDDDPASLTIWVPMFQSSVP
                     ADLLIKELASINILVKQISTPKGPSLRVTINSRSAVLAQMPSNFTISANVSLDERSKL
                     AYDVTTPCEIKACSLTCLKVKSMLTTVKDLTMKTFNPTHEIIALCEFENIMTSKRVII
                     PTYLRSISVKNKDLNSLENIATTEFKNAITNAKIIPYAGLVLVITVTDNKGAFKYIKP
                     QSQFIVDLGAYLEKESIYYVTTNWKHTATRFSIKPLED"
     CDS             4294..4491
                     /note="SH"
                     /codon_start=1
                     /product="small hydrophobic protein"
                     /protein_id="UDY80220.1"
                     /translation="MGNTSITIEFTSKFWPYFTLIHMILTLISLLIIITIMIAILNKL
                     SEHKTFCNETLEQGQMYQINT"
     CDS             4681..5634
                     /note="G"
                     /codon_start=1
                     /product="attachment glycoprotein"
                     /protein_id="UDY80221.1"
                     /translation="MSKNKNQRTARTLEKTWDTLNHLIVISSCLYKLNLKSIAQIALS
                     VLAMIISTSLIIAAIIFIISANHKVTLTTVTVQTIKNHTEKNITTYLTQVSPERVSPS
                     NQPTTTPPIHTNSATISPNTKSETHHTTAQTKGRTSTPTQNNKPNTKPRPKNPPKKDD
                     YHFEVFNFVPCSICGNNQLCKSICKTIPSNKPKKKPTTKPTNKPPTKTTNKRDPKTLA
                     KTPKKETTINPTKKPTPKTTERDTSTPQSTVLDTTTSKHTERDTSTSQSIALDTTTSK
                     HTIQQQSLYSTTPENTPNSTQTPTASEPSTSNSTQKLQSYA"
     CDS             5711..7435
                     /note="F"
                     /codon_start=1
                     /product="fusion protein"
                     /protein_id="UDY80222.1"
                     /translation="MELLIHRSSAIFLTFAINALYLTSSQNITEEFYQSTCSAVSRGY
                     LSALRTGWYTSVITIELSNIKETKCNGTDTKVKLIKQELDKYKNAVTELQLLMQNTPA
                     ANNRARREAPQYMNYTINTTKNLNVSISKKRKRRFLGFLLGVGSAIASGIAVSKVLHL
                     EGEVNKIKNALLSTNKAVVSLSNGVSVLTSKVLDLKNYINNQLLPIVNQQSCRISNIE
                     TVIEFQQKNSRLLEITREFSVNAGVTTPLSTYMLTNSELLSLINDMPITNDQKKLMSS
                     NVQIVRQQSYSIMSIIKEEVLAYVVQLPIYGVIDTPCWKLHTSPLCTTNIKEGSNICL
                     TRTDRGWYCDNAGSVSFFPQADTCKVQSNRVFCDTMNSLTLPSEVSLCNTDIFNSKYD
                     CKIMTSKTDISSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTV
                     SVGNTLYYVNKLEGKNLYVKGEPIINYYDPLVFPSDEFDASISQVNEKINQSLAFIRR
                     SDELLHNVNTGKSTTNIMITAIIIVIIVVLLSLIAIGLLLYCKAKNTPVTLSKDQLSG
                     INNIAFSK"
     CDS             7662..8249
                     /note="M2-1"
                     /codon_start=1
                     /product="matrix protein 2-1"
                     /protein_id="UDY80223.1"
                     /translation="MSRRNPCKFEIRGHCLNGRRCHYSHNYFEWPPHALLVRQNFMLN
                     KILKSMDKSIDTLSEISGAAELDRTEEYALGIVGVLESYIGSINNITKQSACVAMSKL
                     LIEINSDDIKKLRDNEEPNSPKIRVYNTVISYIESNRKNSKQTIHLLKRLPADVLKKT
                     IKNTLDIHKSITISNPKESTVSDQNDQTKNNDITG"
     CDS             8215..8487
                     /note="M2-2"
                     /codon_start=1
                     /product="matrix protein 2-2"
                     /protein_id="UDY80224.1"
                     /translation="MTKPKIMILPDKYPCSISSILISSENMIATFNHKDILQFNHNHL
                     DNHQCLLNHIFDEIHWTPKNLLDATQQFLQHLNIPEDIYTVYILVS"
     CDS             8553..15053
                     /note="L"
                     /codon_start=1
                     /product="RNA-directed RNA polymerase"
                     /protein_id="UDY80225.1"
                     /translation="MDPIINGSSANVYLTDSYLKGVISFSECNALGSYLFNGPYLKND
                     YTNLISRQSPLLEHMNLKKLTITQSLISRYHKGELKLEEPTYFQSLLMTYKSMSSSEQ
                     IATTNLLKKIIRRAIEISDVKVYAILNKLGLKEKDRVKPNNNSGDENSVLTTIIKDDI
                     LSAVENNQSYTNSDKNYSVNQNINIKTTLLKKLMCSMQHPPSWLIHWFNLYTKLNNIL
                     TQYRSNEVKSHGFILIDNQTLSGFQFILNQYGCIVYHKGLKKITTTTYNQFLTWKDIS
                     LSRLNVCLITWISNCLNTLNKSLGLRCGFNNVVLSQLFLYGDCILKLFHNEGFYIIKE
                     VEGFIMSLILNITEEDQFRKRFYNSMLNNITDAAIKAQKDLLSRVCHTLLDKTVSDNI
                     INGKWIILLSKFLKLIKLAGDNNLNNLSELYFLFRIFGHPMVDERQAMDAVRINCNET
                     KFYLLSSLSTLRGAFIYRIIKGFVNTYNRWPTLRNAIVLPLRWLNYYKLNTYPSLLEI
                     TENDLIILSGLRFYREFHLPKKVDLEMIINDKAISPPKDLIWTSFPRNYMPSHIQNYI
                     EHEKLKFSESDRSRRVLEYYLRDNKFNECDLYNCVVNQSYLNNSNHVVSLTGKERELS
                     VGRMFAMQPGMFRQIQILAEKMIAENILQFFPESLTRYGDLELQKILELKAGISNKSN
                     RYNDNYNNYISKCSIITDLSKFNQAFRYETSCICSDVLDELHGVQSLFSWLHLTIPLV
                     TIICTYRHAPPFIKDHVVNLNEVDEQSGLYRYHMGGIEGWCQKLWTIEAISLLDLISL
                     KGKFSITALINGDNQSIDISKPVRLIEGQTHAQADYLLALNSLKLLYKEYAGIGHKLK
                     GTETYISRDMQFMSKTIQHNGVYYPASIKKVLRVGPWINTILDDFKVSLESIGSLTQE
                     LEYRGESLLCSLIFRNIWLYNQIALQLRNHALCNNKLYLDILKVLKHLKTFFNLDSID
                     TALSLYMNLPMLLGGGDPNLLYRSFYRRTPDFLTEAIVHSVFVLSYYTGHDLQDKLQD
                     LPDDRLNKFLTCVITFDKNPNAEFVTLMRDPQALGSERQAKITSEINRLAVTEVLSIA
                     PNKIFSKSAQHYTTTEIDLNDIMQNIEPTYPHGLRVVYESLPFYKAEKIVNLISGTKS
                     ITNILEKTSAIDTTDINRATDMMRKNITLLIRILPLDCNKDKRELLSLENLSITELSK
                     YVRERSWSLSNIVGVTSPSIMFTMDIKYTTSTIASGIIIEKYNVNGLTRGERGPTKPW
                     VGSSTQEKKTMPVYNRQVLTKKQRDQIDLLAKLDWVYASIDNKDEFMEELSTGTLGLS
                     YEKAKKLFPQYLSVNYLHRLTVSSRPCEFPASIPAYRTTNYHFDTSPINHVLTEKYGD
                     EDIDIVFQNCISFGLSLMSVVEQFTNICPNRIILIPKLNEIHLMKPPIFTGDVDIIKL
                     KQVIQKQHMFLPDKISLTQYVELFLSNKALKSGSHINSNLILVHKMSDYFHNAYILST
                     NLAGHWILIIQLMKDSKGIFEKDWGEGYITDHMFINLNVFFNAYKTYLLCFHRGYGKA
                     KLECDMNTSDLLCVLELIDSSYWKSMSKVFLEQKVIKYIVNQDASLHRIKGCHSFKLW
                     FLKRLNNAKFTVCPWVVNIDYHPTHMKAILSYIDLVRMGLINVDKLTIKNKNKFNDEF
                     YTSNLFYISYNFSDNTHLLTKQIRIANSELEDNYNKLYQPTPETLENISLIPVKSNNR
                     NKPKFCISGNTESMMTSTFSNKMHIKSSTVTTRFNYSRQDLYNLFPIVVIDRIIDHSG
                     NTEKSNQLYTTTSHQTSLVRNSASLYCMLPWHHVNRFNFVFSSTGCKISIEYILKDLK
                     IKDPSCIAFIGEGAGNLLLRTVVELHPDIRYIYRSLKDCNDHSLPIEFLRLYNGHINI
                     DYGENLTIPATDATNNIHWSYLHIKFAEPISIFVCDAELPVTTNWSKIIIEWSKHVRK
                     CKYCSSVNRCILIAKYHAQDDIDFKLDNITILKTYVCLGSKLKGSEVYLVLTIGPANI
                     LPVFDVVQNAKLILSRTKNFIMPKKIDKESIDANIKSLIPFLCYPITKNGIKTSLSKL
                     KSVVNGDILSYSIAGRNEVFSNKLINHKHMNILKWLDHVLNFRSAELNYNHLYMIEST
                     YPYLSELLNSLTTNELKKLIKITGSVLYNLPNEQ"
ORIGIN
        1 aaatgcgtac tacaaacttg cacactcgga aaaaatgggg caaataagaa cttgataagt
       61 gctatttaag tctaaccttt ttaatcagaa atggggtgca attcactgag catgataaag
      121 gttagattac aaaatttatt tgacaatgat gaagtagcat tgttaaaaat aacatgttat
      181 actgacaaat taattcttct gactaatgca ttagccaaag caacaataca tacaattaaa
      241 ttaaacggca tagtttttat acatgttata acaagcagtg aagtgtgccc tgataacaat
      301 attgtagtga aatctaactt tacaacaatg ccaatattac aaaatggagg atacatatgg
      361 gagttgattg aattgacaca ctgctctcaa ttaaatggtc taatagatga taattgtgaa
      421 atcaaatttt ctaaaagact aagtgactca gtaatgactg attatatgaa tcaaatatct
      481 gatttacttg ggcttgatct ccattcatga attatgttta gtctaattca atagacatgt
      541 gtttattacc attttagtta atataaaacc tcatcaaagg gaaatggggc aaataaattc
      601 acccaatcaa tcaaaccatg agcactacaa acgacaacac caccatgcaa agattgatga
      661 tcacagacat gagacccctg tcgatggatt caataataac atctctcacc aaagaaatca
      721 tcacacacaa attcatatat ttgataaaca atgaatgtat tgtaagaaaa ctcgatgaga
      781 gacaagctac atttacattc ctagtcaatt atgagatgaa gctattgcac aaagtaggga
      841 gtaccaaata caagaaatac actgaatata atacaaaata tggcacattc cctatgccta
      901 tatttatcaa tcatggcggg tttctagaat gtattggcat taagcctaca aaacatactc
      961 ctataatata caaatatgac ctcaacccgt aacttccaac aaaaaaacca actcacccaa
     1021 accaagctat tctctaaaca acagtgctca acagttaaga agaagctaat ccattttagt
     1081 aattaaaaat aagggtgaag ccagtaacat aaattggggc aaatacaaag atggctctta
     1141 gcaaagtcaa gttgaatgat acattaaata aggatcagtt gctgtcatcc agcaaataca
     1201 ctattcaacg tagtacagga gataatattg acactcccaa ttatgatgtg caaaaacacc
     1261 taaacaaact atgtggtatg ctattaatca ctgaagatgc aaatcataaa tttacaggat
     1321 taataggtat gctatatgct atgtccaggt taggaaggga agacactata aagatactta
     1381 aagatgctgg atatcatgtt aaagctaatg gagtagatat aacaacatat cgtcaagata
     1441 taaatggaaa ggaaatgaaa ttcgaagtat taacattatc aagcttgaca tcagaaatac
     1501 aagtcaatat tgagatagaa tctagaaagt cctacaaaaa aatgctaaaa gagatgggag
     1561 aagtggctcc agaatatagg catgattctc cagactgtgg gatgataata ctatgtatag
     1621 ctgcccttgt aataaccaaa ttagcagcag gagatagatc aggtcttaca gcagtaatta
     1681 ggagggcaaa caatgtctta aaaaacgaaa taaaacgcta caagggcctg ataccaaagg
     1741 acatagccaa cagtttttat gaagtgtttg aaaaacaccc tcatcttata gatgtttttg
     1801 tgcactttgg cattgcacaa tcatccacaa gagggggtag tagagttgaa ggaatctttg
     1861 caggattgtt tatgaatgcc tatggttcag gacaagtaat gctaagatgg ggagttttag
     1921 ccaaatctgt aaaaaatatc atgctaggac atgctagtgt ccaagcagaa atggagcaag
     1981 ttgtggaagt ctatgagtat gcacagaagt tgggaggaga agctggtttc taccatatat
     2041 tgaacaatcc aaaagcatca ttgctgtcat taactcaatt tcctaacttc tcaagtgtgg
     2101 tcctaggcaa tgcagcaggt ctaggcataa tgggagagta tagaggtaca ccaagaaacc
     2161 aagatctcta tgatgcagcc aaagcatatg cagagcaact caaagaaaat ggagtaataa
     2221 actacagtgt attagactta acaacagaag aattggaagc cataaagcat caactcaacc
     2281 ccaaagaaga tgacgtagag ctttaagtta acaaaaaata cggggcaaat aagtcaacat
     2341 ggagaagttt gcacctgaat ttcatggaga agatgcaaat aacaaggcta ccaaattcct
     2401 agaatcaata aaaggcaagt ttgcatcatc caaagatcct aagaagaaag atagcataat
     2461 atctgtcaac tcaatagaca tagaagtcac taaagagagc ccaataacat ctggcaccaa
     2521 cattatcaat ccaacaagcg aagctgacag taccctagaa accaaagcca actacccaag
     2581 aaaaccccta gtaagcttca aagaagatct caccccaagt gataaccctt tctccaagtt
     2641 gtacaaagaa accatagaaa catttgataa caatgaagaa gaatctagct actcatatga
     2701 ggagatcaat gatcaaacaa atgacaacat tacagcaaga ctagatagaa ttgatgaaaa
     2761 attaagtgaa atattaggaa tgctccatac attagtagtt gcaagtgctg gacccacttc
     2821 ggctcgtgat ggaataagag atgctatggt tggtctaaga gaagaaatga tagaaaaaat
     2881 aagagcagaa gcattaatga ccaatgatag gttagaggct atggcaagac ttaggaatga
     2941 ggaaagcgaa aagatgacaa aagacacctc agatgaagtg tctctcaatc caacctccaa
     3001 aaaattgagt gacttgttag aagacaacga tagcgacaat gatctatcac ttgatgattt
     3061 ttgatcagtg atcaactcac tcagcaatca acaacatcaa taaaacagac accaatccat
     3121 tgaatcaatt gccagactga aaaaacaacc atccatcagc agaaccacca accaatcgat
     3181 caaccaattg atcaatcagc accctgacga aattaacaat atagtaacaa aaaaaggaac
     3241 aagatggggc aaatatggaa acatacgtga acaagcttca cgaaggctcc acatacacag
     3301 cagctgttca gtacaatgtt ctagaaaaag atgatgatcc cgcatcacta acaatatggg
     3361 tgcctatgtt ccagtcatct gtgccagcag acttgctcat aaaagaactt gcaagcatca
     3421 acatactagt aaagcagatc tctacgccca aaggaccttc actacgggtc acaatcaact
     3481 caagaagtgc tgtgctggct caaatgccta gtaatttcac cataagtgca aatgtatcat
     3541 tagatgaaag aagcaaatta gcatatgatg taactacacc ttgtgaaatc aaagcatgca
     3601 gtctaacatg cttaaaagta aaaagtatgt taactacagt caaagatcta accatgaaga
     3661 cattcaaccc cactcatgag atcattgctc tatgtgaatt tgaaaatatt atgacatcaa
     3721 aaagagtaat aataccaacc tatctaagat caattagtgt caaaaacaag gacctgaact
     3781 cactagaaaa tatagcaacc actgaattca aaaatgctat caccaatgct aaaattattc
     3841 cctatgcggg attagtattg gttatcacgg ttactgataa caaaggagca ttcaaatata
     3901 tcaagccaca gagtcaattc atagtggatc ttggcgccta cctagaaaaa gagagcatat
     3961 attatgtgac taccaattgg aagcacacag ctacacgctt ttcaatcaaa ccactagagg
     4021 attaaaccaa attatcaaca ttgaatgaca gattcacata catcctcaac tgcacactat
     4081 atctaaacat cataaacatc cacactacac acttcatcac acaaaccaat cccactcaaa
     4141 atccaaaatc acttccagcc attgtctgcc agacatagag tgtgaatagg taaataaaac
     4201 cagaatatgg ggtaaataga tatcagttag agttcaatca atctcaacaa ccatctatac
     4261 cgccaattca atacatatac tgcaaatctc aaaatgggaa acacatccat cacaatagaa
     4321 ttcacaagca aattttggcc ctattttaca ctaatacata tgatcttaac tctaatctcc
     4381 ttactaatta taatcactat tatgattgca atactaaata agctaagtga acataaaaca
     4441 ttctgtaacg aaactcttga acaaggacag atgtatcaaa tcaacacata gtgttctccc
     4501 attatgctgt gtcaaattat aatcttgtat atataaataa acaaatccaa tcttctcata
     4561 gagtcatggt atcacaaaac catgccaacc attatggtag catagagtag ttatttaaaa
     4621 attaacataa tgatgaatta ttagtatggg atcaaaaaca acattggggc aaatgcaacc
     4681 atgtccaaaa acaagaatca acgcactgcc aggactctag aaaagacctg ggatactctt
     4741 aatcatctaa ttgtaatatc ctcttgttta tacaaattaa atttaaaatc tatagcacaa
     4801 atagcactat cagttttggc aatgataatc tcaacctctc tcataattgc agccataata
     4861 ttcatcatct ctgctaatca caaagttaca ctaacaactg tcacagttca aacaataaaa
     4921 aaccacactg agaaaaacat aaccacttac cttactcaag tctcaccaga aagggttagc
     4981 ccatccaacc aacccacaac cacaccacca atccacacaa actcagccac aatatcacct
     5041 aatacaaaat cagaaacaca ccatacaaca gcacaaacca aaggcagaac ctctactcca
     5101 acacagaaca acaagccaaa cacaaaacca cgtccaaaaa atccaccaaa aaaagatgat
     5161 taccattttg aagtgttcaa cttcgttccc tgtagtatat gtggcaacaa tcaactctgc
     5221 aaatccatct gcaaaacaat accaagcaat aaaccaaaga aaaaaccaac cacaaaaccc
     5281 acaaacaaac cacccaccaa aaccacaaac aaaagagacc ccaaaacact agccaaaaca
     5341 ccgaaaaaag aaaccaccat taacccaaca aaaaaaccaa cccccaagac cacagaaaga
     5401 gacaccagca ccccacaatc cactgtgctc gacacaacca catcgaaaca cacagaaaga
     5461 gacaccagca cctcacaatc cattgcgctt gacacaacca catcaaaaca cacaatccaa
     5521 cagcaatccc tctactcaac cacccccgaa aacacaccaa actccacaca aacacccaca
     5581 gcatccgagc cctctacatc aaattccacc caaaaactcc agtcatatgc ttagttattt
     5641 aaaaactaca tcttagcaga gaaccgtgat ccctcaagca agaacgaaat taaatctggg
     5701 gcaaataacc atggagttgc tgatccatag atcaagtgca atcttcctaa cttttgctat
     5761 taatgcattg tacctcacct caagtcagaa cataactgag gagttttacc aatcgacatg
     5821 tagtgcagtt agcagaggtt acttgagtgc tttaagaaca ggttggtata ccagtgtcat
     5881 aacgatagaa ttaagtaata taaaagaaac caaatgcaat ggaactgaca ctaaagtaaa
     5941 acttataaaa caagaattag ataagtataa gaatgcagta acagaattac agttacttat
     6001 gcaaaacaca ccagctgcca acaaccgggc tagaagagaa gcaccacagt atatgaacta
     6061 cacaatcaat accactaaaa acctaaatgt atcaataagc aaaaagagga aacgaagatt
     6121 tctgggcttc ttattaggtg taggatctgc aatagcaagt ggtatagctg tatccaaagt
     6181 tctacacctt gaaggagaag tgaacaagat caaaaatgct ttgctgtcta caaacaaagc
     6241 tgtagtcagt ctatcaaatg gggtcagtgt tttaaccagc aaagtgttag atctcaagaa
     6301 ttatataaac aaccaattat tacctatagt aaatcaacaa agttgtcgca tttccaacat
     6361 tgaaacagtt atagaattcc agcagaagaa cagcagattg ttggaaatca ccagagaatt
     6421 tagtgtcaat gcaggtgtaa caacaccttt aagcacctac atgttaacaa acagtgagtt
     6481 actatcatta atcaatgaca tgcctataac aaatgatcag aaaaaattaa tgtcaagcaa
     6541 tgttcagata gtaaggcaac aaagttattc tatcatgtct ataataaagg aagaagtcct
     6601 tgcatatgtt gtacagctac ctatctatgg tgtaattgat acaccttgct ggaaattaca
     6661 cacatcacct ctgtgcacca ccaacatcaa agaaggatca aatatttgtt taacaaggac
     6721 tgatagagga tggtactgtg ataatgcagg atcagtatcc ttctttccgc aggctgacac
     6781 ttgtaaagta cagtccaatc gagtattttg tgacactatg aacagtttga cattaccaag
     6841 tgaagtcagc ctttgtaaca ctgacatatt caattccaag tatgactgca aaattatgac
     6901 atcaaaaaca gacataagca gctcagtaat tacttctcta ggagctatag tgtcatgcta
     6961 tggtaaaact aaatgcactg catccaacaa aaatcgtgga attataaaga cattttctaa
     7021 tggttgtgat tatgtgtcaa acaaaggagt agatactgta tcagtgggca acactttata
     7081 ctatgtcaac aagctggaag gaaaaaacct ttatgtaaaa ggggaaccta taataaatta
     7141 ctatgaccct ctagtgtttc cttctgatga gtttgatgca tcaatatctc aagtcaatga
     7201 aaaaattaat caaagtttag cttttattcg tagatccgat gaattattac ataatgtaaa
     7261 cactggaaaa tctactacaa atattatgat aactgcaatt attatagtaa tcattgtagt
     7321 attgttatca ttaatagcta ttggtttact gttgtattgc aaagccaaaa acacaccagt
     7381 tacactaagc aaagaccaac taagtggaat caataacatt gcattcagca aatagacaaa
     7441 aaaccacctg atcatgtttc aacaacaatc tgctgaccac caatcccaaa tcaacttaca
     7501 acaaatactt caacatcaca gcacaggctg aatcattccc tcgcatcatg atacccacac
     7561 aactaagcta gatccttaac tcatagttat ataaaaccct caaatatcac aatcaacact
     7621 aaatcaacac atcattcaca aaactaacag ctggggcaaa tatgtcgcgg agaaatccct
     7681 gcaaatttga gattagaggt cattgcttga atggtagaag atgccactac agtcataatt
     7741 actttgaatg gcctcctcat gcattgctag tgaggcaaaa cttcatgtta aacaagatac
     7801 ttaagtcaat ggacaagagc atagacactt tgtctgaaat aagtggagct gctgaacttg
     7861 atagaacaga agaatatgct cttggtatag ttggagtgct agagagttac ataggatcta
     7921 taaacaacat aacaaaacaa tcagcatgtg ttgctatgag taaacttctt attgagatca
     7981 atagtgatga cattaaaaag ctgagagaca atgaagaacc caattcacct aagataagag
     8041 tatacaatac tgttatatca tacatcgaga gcaatagaaa aaacagcaag caaaccatcc
     8101 atctactcaa acgattacca gcagacgtgc tgaagaagac aataaagaac acattagata
     8161 tccacaaaag cataaccata agcaacccaa aagagtcaac cgtaagtgat caaaatgacc
     8221 aaaccaaaaa taatgatatt accggataaa tatccttgta gtatatcatc catattgatc
     8281 tcaagtgaaa acatgattgc tacattcaat cataaagaca tattacaatt taaccacaac
     8341 catttggata atcaccagtg tttattaaat catatatttg atgaaattca ttggacacct
     8401 aaaaacttat tagatgccac tcaacaattt ctccaacatc ttaacatccc tgaagatata
     8461 tatacagtat atatattagt atcataatgc ttgatcataa cgattctata tcatccagcc
     8521 ataaaactgt cttaataagg ttatgggaca aaatggatcc cattattaat ggaagctctg
     8581 ctaatgtata tctaactgat agttatctaa aaggtgttat ctctttttca gaatgtaatg
     8641 ctttagggag ttaccttttt aacggccctt atcttaaaaa tgattatacc aacttaatta
     8701 gtagacaaag cccactacta gagcatatga atctaaaaaa actaactata acacagtcat
     8761 taatatctag ataccataaa ggtgaactga aattagaaga accaacttat ttccagtcat
     8821 tacttatgac atataaaagc atgtcctcgt ctgaacaaat tgctacaact aacttactta
     8881 aaaaaataat acgaagagct atagaaataa gtgatgtaaa ggtgtacgcc atcttgaata
     8941 aactaggact aaaggaaaag gacagagtta agcccaacaa caattcaggt gatgaaaact
     9001 cagtacttac aaccataatt aaagatgata tactctcagc tgtggaaaac aatcaatcat
     9061 atacaaattc agacaaaaat tactcagtaa atcaaaatat caatatcaaa acaacactct
     9121 taaaaaaatt gatgtgttca atgcaacatc ctccatcatg gttaatacac tggttcaatt
     9181 tatatacaaa attaaataac atattaacac aatatcgatc aaatgaggta aaaagtcatg
     9241 ggtttatatt aatagataat caaactttga gtggttttca gtttatttta aatcaatatg
     9301 gttgcattgt ttatcataaa gggctcaaaa aaatcacaac tactacatac aatcaatttt
     9361 tgacatggaa agacatcagc cttagcagat taaatgtttg cttaattact tggataagta
     9421 attgtttaaa tacattaaat aaaagcttag ggttgagatg cggattcaat aatgttgtgc
     9481 tatcacaatt attcctgtat ggagattgta tactgaaatt atttcataat gagggctttt
     9541 acataataaa agaagtagaa ggatttatta tgtctttaat tctaaacata acagaagaag
     9601 atcaatttag gaaacgattt tataatagca tgctaaataa catcacagat gcagctatta
     9661 aggctcaaaa ggacctacta tcaagagtat gtcacacttt attagacaag acagtgtctg
     9721 ataatatcat aaatggtaaa tggataattc tattaagtaa atttcttaaa ttgattaagc
     9781 ttgcaggtga taataatctc aataacttga gtgagctata ttttctcttc agaatctttg
     9841 gacatccaat ggttgatgaa agacaagcaa tggatgctgt aagaattaac tgcaatgaaa
     9901 ctaagttcta tttattaagt agcctaagta cgttgagagg tgctttcatt tatagaatca
     9961 taaaagggtt tgtaaatact tacaacagat ggcccacttt aaggaatgct attgtcctac
    10021 ctctaagatg gttaaactat tataaactta atacttatcc atctttactt gaaatcacag
    10081 aaaatgattt gattatttta tcaggattgc ggttctatcg tgagtttcat ctgcctaaaa
    10141 aagtggatct tgaaatgata ataaatgaca aagctatttc tcctccaaaa gatctaatat
    10201 ggactagttt tcctagaaat tacatgccat cacatataca aaattatata gaacatgaaa
    10261 agttgaagtt ctctgaaagc gacagatcaa gaagagtact agagtattac ttgagagata
    10321 ataaattcaa tgaatgtgat ctatacaatt gtgtagttaa tcaaagctat ctcaacaact
    10381 ctaatcatgt ggtatcacta actggtaaag aaagagagct cagtgtgggt agaatgtttg
    10441 ctatgcaacc aggtatgttt aggcaaatcc aaatcttagc agagaaaatg atagccgaaa
    10501 atattttaca attcttccct gagagtttga caagatatgg tgatctagag cttcaaaaga
    10561 tattagaatt aaaagcagga ataagcaaca agtcaaatcg ttataatgat aactacaaca
    10621 attatatcag taaatgttct ataataacag atcttagcaa atttaatcaa gcatttagat
    10681 atgaaacatc atgtatctgc agtgatgtat tagatgaact gcatggagta caatctctat
    10741 tctcttggtt gcatttaaca atacctcttg tcacaataat atgtacatat agacatgcac
    10801 ctccttttat aaaggatcat gttgtcaatc ttaatgaagt tgatgaacaa agtgggttgt
    10861 acagatatca tatgggtggt attgagggct ggtgtcaaaa actgtggacc attgaagcca
    10921 tatcattatt agatctaata tctcttaaag gtaaattctc catcacagct ctgataaatg
    10981 gtgataatca gtcaattgat ataagtaaac cagttagact tatagagggt cagacccatg
    11041 ctcaagcaga ttatttgtta gcattaaata gccttaaatt gctatataaa gagtatgcag
    11101 gcataggcca taagcttaag ggaactgaga cctatatatc ccgagatatg cagttcatga
    11161 gcaaaactat ccagcacaat ggagtgtact atccagccag tatcaaaaaa gtcctgagag
    11221 taggtccatg gataaataca atacttgatg attttaaagt tagtttagaa tctataggta
    11281 gcttaacaca ggagttagaa tacagaggag aaagcttatt atgcagttta atatttagga
    11341 acatttggtt atataatcaa attgctctgc aactccgaaa tcatgcatta tgtaataata
    11401 agctatattt agatatcttg aaagtattaa aacacttaaa aacctttttt aatcttgata
    11461 gtatcgatac ggcgttatca ttgtatatga acttgcctat gctacttggt ggtggtgatc
    11521 ctaatttgtt atatcgaagc ttttatagga gaactccaga cttccttaca gaagctatag
    11581 tacattcagt gttcgtgttg agctattata ctggtcacga tctacaagat aagctccagg
    11641 atcttccaga tgatagactg aacaaattct tgacttgtgt catcacattt gataaaaatc
    11701 caaatgccga gtttgtaaca ttgatgaggg atccacaggc tttagggtct gaaaggcaag
    11761 ctaaaattac tagtgagatt aatagattag cagtaacaga agtcttaagt atagctccaa
    11821 acaaaatatt ttctaaaagt gcgcaacact atactaccac tgagattgat ctaaatgaca
    11881 ttatgcaaaa tatagaacca acttaccctc atggattaag agttgtttat gaaagtttac
    11941 ctttttataa agcagaaaaa atagttaatc ttatatcagg aacaaaatcc ataactaata
    12001 tacttgaaaa aacatcagcg atagatacaa ctgatattaa tagggctact gatatgatga
    12061 gaaaaaatat aaccttactt ataaggatac ttccactaga ttgtaacaag gacaaaagag
    12121 agttattaag tttagaaaat cttagcataa ctgaattaag caagtatgta agagaaagat
    12181 cttggtcatt atccaatata gtaggagtaa catcgccaag tattatgttc acaatggaca
    12241 ttaaatatac aactagcact atagccagtg gtataattat agaaaaatat aatgttaatg
    12301 gtttaactcg tggtgaaaga ggacctacta agccatgggt aggttcatct acgcaggaga
    12361 aaaaaacaat gccagtgtac aatagacaag ttttaaccaa aaagcaaaga gaccaaatag
    12421 atttattagc aaaattagac tgggtatatg catccataga caacaaagat gaattcatgg
    12481 aagaactaag tactggaaca cttggactgt catatgaaaa agccaaaaag ttgtttccac
    12541 aatatctaag tgtcaattat ttacaccggt taacagtcag tagtagacca tgcgaattcc
    12601 ctgcctctat accagcttat agaacaacaa attatcattt tgatactagt cctatcaatc
    12661 atgtattaac agaaaagtat ggagatgaag atattgacat tgtgtttcaa aattgcataa
    12721 gttttggtct tagtttgatg tcagttgtgg aacaattcac aaacatatgt cctaatagaa
    12781 ttattctcat accgaagctg aatgagatac atttgatgaa accccctata tttacaggag
    12841 atgttgatat catcaagttg aagcaagtga tacaaaaaca gcatatgttc ctaccagata
    12901 aaataagttt aacccaatat gtagaattat tcttaagtaa caaagcactt aaatctggat
    12961 cccacatcaa ctctaattta atattagtac ataaaatgtc tgattatttt cataatgcgt
    13021 atattttaag tactaattta gctggacatt ggattctgat tattcaactt atgaaagatt
    13081 caaaaggtat ttttgaaaaa gattggggag aagggtatat aactgatcat atgttcatta
    13141 atttgaatgt tttctttaat gcttataaga cttatttgct atgttttcat agaggttatg
    13201 gtaaagcgaa attagaatgt gatatgaaca cttcagatct tctttgtgtt ttggagttaa
    13261 tagacagtag ctactggaaa tctatgtcta aagttttcct ggaacaaaaa gtcataaaat
    13321 acatagtcaa tcaagacgca agtttgcata gaataaaagg ctgtcacagt tttaagttgt
    13381 ggtttttaaa acgccttaat aatgctaaat ttaccgtatg cccctgggtt gttaacatag
    13441 attatcaccc aacacacatg aaagctatat tatcttacat agatttagtt agaatggggt
    13501 taataaatgt agataaatta accattaaaa ataaaaacaa attcaatgat gaattttaca
    13561 catcaaatct cttttacatt agttataact tttcagacaa cactcatctg ctaacaaaac
    13621 aaataagaat tgctaattca gaattagaag ataattataa caaactatat caaccaaccc
    13681 cagaaacttt agaaaatata tcattaatcc ctgttaaaag taataataga aacaaaccta
    13741 aattttgtat aagtggaaat actgaatcta tgatgacgtc aacattctct aataaaatgc
    13801 atattaaatc ttccactgtt accacaagat tcaattatag cagacaagac ttgtacaatt
    13861 tatttccaat tgttgtgata gacaggatta tagatcattc aggtaataca gaaaaatcta
    13921 accaacttta caccaccact tcacatcaga catctttagt aaggaatagt gcatcacttt
    13981 attgcatgct tccttggcat catgtcaata gatttaactt tgtatttagt tccacaggat
    14041 gcaagatcag tatagaatat attttaaaag atctcaagat taaagatccc agttgtatag
    14101 cattcatagg tgaaggagct ggtaacttat tattacgtac ggtagtagaa cttcatcctg
    14161 acataagata tatttacaga agtttaaaag attgcaatga tcatagttta cctattgaat
    14221 ttctaaggtt atacaacggg catataaaca tagattatgg tgagaattta accattcctg
    14281 ctacagatgc aactaacaac atacattggt cttatttaca tataaaattt gcagaaccta
    14341 ttagtatctt tgtctgcgat gctgaattac ctgtcacaac caattggagt aaaattataa
    14401 ttgaatggag taagcatgta agaaagtgca agtactgttc ttctgtaaat agatgcattt
    14461 taattgcaaa atatcatgct caagatgata ttgatttcaa attagataac attactatat
    14521 taaaaactta tgtgtgccta ggtagcaagt taaaaggatc tgaagtttat ttagtcctta
    14581 caataggccc tgcaaatata cttcctgttt ttgatgttgt gcaaaatgct aaattgattc
    14641 tttcaagaac taaaaatttc attatgccta aaaagattga caaggaatct atcgatgcaa
    14701 atattaaaag cttaatacct ttcctttgtt accctataac aaaaaatgga attaagactt
    14761 cattgtcaaa attgaagagt gtagttaatg gagatatatt atcatattct atagctggac
    14821 gtaatgaagt attcagcaac aagcttataa accacaagca tatgaatatc ttaaaatggc
    14881 tggatcatgt tttaaacttt agatcagctg aacttaatta caatcattta tacatgatag
    14941 agtctacata tccttacttg agtgaattgt taaatagttt aacaaccaat gagctcaaga
    15001 agctgattaa aataacaggt agtgtactat acaaccttcc taatgagcag taactcaaaa
    15061 tatcattaac aagtttggtc aactttagat gctaacacat cattatatta tagttattaa
    15121 aaaatatgca aacttttcaa taatttagca tattgattcc aaaattacca ttttagtctt
    15181 aagggattaa ataaaagtct aaaactaaca attacacatg tgcatttaca acacaacgag
    15241 acattagttt t
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