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pAc5. 1V5-His B 质粒 DNA

发布时间：2024/6/18 14:36:13 阅读次数：77

CAT#:JW-1-287
低温运输，-20℃保存

产品及特点
本产品为 pAc5. 1V5-His B 质粒 DNA ，是具有 P-AC5 (Actin 5C)启动子的果蝇表达载体，可用于以果蝇细胞为宿主的重组蛋白的高水平瞬时表达。pAc5. 1V5-His B 质粒 DNA 具有多克隆位点，方便用户在不同酶切位点插入所选基因进行研究或表达。

本质粒携带 Amp 抗性基因。其图谱如下：

规格及成分本产品使用塑料袋包装
       成分    编号              规格        包装
pAc5. 1V5-His B 质粒 DNA 溶液（浓度见标签）   1-287      2 μg       0.5 mL 本色管
使用手册 1-287sc      1 份    无

运输及保存   低温运输，-20℃保存，有效期两年。
使用方法   pAc5. 1V5-His B 质粒 DNA 溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒 DNA 进行各种后续实验。

DNA 序列
     1       CTTCAAGGAA        TTAATTCTAT           ATTCTAAAAA           CACAAATGAT           ACTTCTAAAA 50
     51     AAAATCATGA        ATGGCATCAA           CTCTGAATCA           AATCTTTGCA            GATGCACCTA 100
    101    CTTCTCATTT           CCACTGTCAC             ATCATTTTTC              CAGATCTCGC         TGCCTGTTAT 150
    151    GTGGCCCACA         AACCAAGACA            CGTTTTATGG            CCATTAAAGC         TGGCTGATCG+ 200
    201     TCGCCAAACA       CCAAATACAT          ATCAATATGT          ACATTCGAGA          AAGAAGCGAT 250
    251     CAAAGAAGCG        TCTTCGGGCG           AGTAGGAGAA           TGCGGAGGAG       AAGGAGAACG 300
     301    AGCTGATCTA        GTATCTCTCC            ACAATCCAAT          GCCAACTGAC         CAACTGGCCA 350
    351     TATTCGGAGC       AATTTGAAGC        CAATTTCCAT        CGCCTGGCGA         TCGCTCCATT 400
    401     CTTGGCTATA     TGTTTTTCAC     CGTTCCCGGG        GCCATTTTCA     AAGACTCGTC 450
   451   GGTAAGATAA   GATTGTGTCA   CTCGCTGTCT   CTCTTCATTT   GTCGAAGAAT   500
   501   GCTGAGGAAT   TTCGCGATGA   CGTCGGCGAG   TATTTTGAAG   AATGAGAATA   550
   551   ATTTGTATTT   ATACGAAAAT   CAGTTAGTGG   AATTTTCTAC   AAAAACATGT   600
   601   TATCTATAGA   TAATTTTGTT   GCAAAATATG   TTGACTATGA   CAAAGATTGT   650
   651   ATGTATATAC   CTTTAATGTA   TTCTCATTTT   CTTATGTATT   TATAATGGCA   700
   701   ATGATGATAC   TGATGATATT   TTAAGATGAT   GCCAGACCAC   AGGCTGATTT   750
   751   CTGCGTCTTT   TGCCGAACGC   AGTGCATGTG   CGGTTGTTGT   TTTTTGGAAT   800
   801   AGTTTCAATT   TTCGGACTGT   CCGCTTTGAT   TTCAGTTTCT   TGGCTTATTC   850
   851   AAAAAGCAAA   GTAAAGCCAA   AAAAGCGAGA   TGGCAATACC   AAATGCGGCA   900
   901   AAACGGTAGT   GGAAGGAAAG   GGGTGCGGGG   CAGCGGAAGG   AAGGGTGGGG   950
   951   CGGGGCGTGG   CGGGGTCTGT   GGCTGGGCGC   GACGTCACCG   ACGTTGGAGC   1000
   1001   CACTCCTTTG   ACCATGTGTG   CGTGTGTGTA   TTATTCGTGT   CTCGCCACTC   1050
   1051   GCCGGTTGTT   TTTTTCTTTT   TATCTCGCTC   TCTCTAGCGC   CATCTCGTAC   1100
   1101   GCATGCTCAA   CGCACCGCAT   GTTGCCGTGT   CCTTTATGCG   TCATTTTGGC   1150
   1151   TCGAAATAGG   CAATTATTTA   AACAAAGATT   AGTCAACGAA   AACGCTAAAA   1200
   1201   TAAATAAGTC   TACAATATGG   TTACTTATTG   CCATGTGTGT   GCAGCCAACG   1250
   1251   ATAGCAACAA   AAGCAACAAC   ACAGTGGCTT   TCCCTCTTTC   ACTTTTTGTT   1300
   1301   TGCAAGCGCG   TGCGAGCAAG   ACGGCACGAC   CGGCAAACGC   AATTACGCTG   1350
   1351   ACAAAGAGCA   GACGAAGTTT   TGGCCGAAAA   ACATCAAGGC   GCCTGATACG   1400
   1401   AATGCATTTG   CAATAACAAT   TGCGATATTT   AATATTGTTT   ATGAAGCTGT   1450
   1451   TTGACTTCAA   AACACACAAA   AAAAAAAATA   AAACAAATTA   TTTGAAAGAG   1500
   1501   AATTAGGAAT   CGGACAGCTT   ATCGTTACGG   GCTAACAGCA   CACCGAGACG   1550
   1551   AAATAGCTTA   CCTGACGTCA   CAGCCTCTGG   AAGAACTGCC   GCCAAGCAGA   1600
   1601   GAGAGAGAGA   AAAAGAGGGA   GAGCAGCTTA   GACCGCATGT   GCTTGTGTGT   1650
   1651   GAGGCGTCTC   TCTCTTCGTC   TCCTGTTTGC   GCAAACGCAT   AGACTGCACT   1700
   1701   GAGAAAATCG   ATTACCTATT   TTTTATGAAT   GAATATTTGC   ACTATTACTA   1750
   1751   TTCAAAACTA   TTAAGATAGC   AATCACATTC   AATAGCCAAA   TACTATACCA   1800
   1801   CCTGAGCGAT   GCAACGAAAT   GATCAATTTG   AGCAAAAATG   CTGCATATTT   1850
   1851   AGGACGGCAT   CATTATAGAA   ATGCTTCTTG   CTGTGTACTT   TTCTCTCGTC   1900
   1901   TGGCAGCTGT   TTCGCCGTTA   TTGTTAAAAC   CGGCTTAAGT   TAGGTGTGTT   1950
   1951   TTCTACGACT   AGTGATGCCC   CTACTAGAAG   ATGTGTGTTG   CACAAATGTC   2000
   2001   CCTGAATAAC   CAATTTGAAG   TGCAGATAGC   AGTAAACGTA   AGCTAATATG   2050
   2051   AATATTATTT   AACTGTAATG   TTTTAATATC   GCTGGACATT   ACTAATAAAC   2100
   2101   CCACTATAAA   CACATGTACA   TATGTATGTT   TTGGCATACA   ATGAGTAGTT   2150
   2151   GGGGAAAAAA   TGTGTAAAAG   CACCGTGACC   ATCACAGCAT   AAAGATAACC   2200
   2201   AGCTGAAGTA   TCGAATATGA   GTAACCCCCA   AATTGAATCA   CATGCCGCAA   2250
   2251   CTGATAGGAC   CCATGGAAGT   ACACTCTTCA   TGGCGATATA   CAAGACACAC   2300
   2301   ACAAGCACGA   ACACCCAGTT   GCGGAGGAAA   TTCTCCGTAA   ATGAAAACCC   2350
   2351   AATCGGCGAA   CAATTCATAC   CCATATATGG   TAAAAGTTTT   GAACGCGACT   2400
   2401   TGAGAGCGGA   GAGCATTGCG   GCTGATAAGG   TTTTAGCGCT   AAGCGGGCTT   2450
   2451   TATAAAACGG   GCTGCGGGAC   CAGTTTTCAT   ATCACTACCG   TTTGAGTTCT   2500
   2501   TGTGCTGTGT   GGATACTCCT   CCCGACACAA   AGCCGCTCCA   TCAGCCAGCA   2550
   2551   GTCGTCTAAT   CCAGAGACCC   CGGATCGGGG   TACCTACTAG   TCCAGTGTGG   2600
   2601   TGGAATTCTG   CAGATATCCA   GCACAGTGGC   GGCCGCTCGA   GTCTAGAGGG   2650
   2651   CCCGCGGTTC   GAAGGTAAGC   CTATCCCTAA   CCCTCTCCTC   GGTCTCGATT   2700
   2701   CTACGCGTAC   CGGTCATCAT   CACCATCACC   ATTGAGTTTA   AACCCGCTGA   2750
   2751   TCAGCCTCGA   CTGTGCCTTC   TAAGGCCTGA   GCTCGCTGAT   CAGCCTCGAT   2800
   2801   CGAGGATCCA   GACATGATAA   GATACATTGA   TGAGTTTGGA   CAAACCACAA   2850
   2851   CTAGAATGCA   GTGAAAAAAA   TGCTTTATTT   GTGAAATTTG   TGATGCTATT   2900
   2901   GCTTTATTTG   TAACCATTAT   AAGCTGCAAT   AAACAAGTTA   ACAACAACAA   2950
   2951   TTGCATTCAT   TTTATGTTTC   AGGTTCAGGG   GGAGGTGTGG   GAGGTTTTTT   3000
   3001   AAAGCAAGTA   AAACCTCTAC   AAATGTGGTA   TGGCTGATTA   TGATCAGTCG   3050
   3051   ACCGATGCCC   TTGAGAGCCT   TCAACCCAGT   CAGCTCCTTC   CGGTGGGCGC   3100
   3101   GGGGCATGAC   TATCGTCGCC   GCACTTATGA   CTGTCTTCTT   TATCATGCAA   3150
   3151   CTCGTAGGAC   AGGTGCCGGC   AGCGCTCTGG   GTCATTTTCG   GCGAGGACCG   3200
   3201   CTTTCGCTGG   AGCGCGACGA   TGATCGGCCT   GTCGCTTGCG   GTATTCGGAA   3250
   3251   TCTTGCACGC   CCTCGCTCAA   GCCTTCGTCA   CTGGTCCCGC   CACCAAACGT   3300
   3301   TTCGGCGAGA   AGCAGGCCAT   TATCGCCGGC   ATGGCGGCCG   ACGCGCTGGG   3350
   3351   CTACGTCTTG   CTGGCGTTCG   CGACGCGAGG   CTGGATGGCC   TTCCCCATTA   3400
   3401   TGATTCTTCT   CGCTTCCGGC   GGCATCGGGA   TGCCCGCGTT   GCAGGCCATG   3450
   3451   CTGTCCAGGC   AGGTAGATGA   CGACCATCAG   GGACAGCTTC   AAGGATCGCT   3500
   3501   CGCGGCTCTT   ACCAGCCAGC   AAAAGGCCAG   GAACCGTAAA   AAGGCCGCGT   3550
   3551   TGCTGGCGTT   TTTCCATAGG   CTCCGCCCCC   CTGACGAGCA   TCACAAAAAT   3600
   3601   CGACGCTCAA   GTCAGAGGTG   GCGAAACCCG   ACAGGACTAT   AAAGATACCA   3650
   3651   GGCGTTTCCC   CCTGGAAGCT   CCCTCGTGCG   CTCTCCTGTT   CCGACCCTGC   3700
   3701   CGCTTACCGG   ATACCTGTCC   GCCTTTCTCC   CTTCGGGAAG   CGTGGCGCTT   3750
   3751   TCTCAATGCT   CACGCTGTAG   GTATCTCAGT   TCGGTGTAGG   TCGTTCGCTC   3800
   3801   CAAGCTGGGC   TGTGTGCACG   AACCCCCCGT   TCAGCCCGAC   CGCTGCGCCT   3850
   3851   TATCCGGTAA   CTATCGTCTT   GAGTCCAACC   CGGTAAGACA   CGACTTATCG   3900
   3901   CCACTGGCAG   CAGCCACTGG   TAACAGGATT   AGCAGAGCGA   GGTATGTAGG   3950
   3951   CGGTGCTACA   GAGTTCTTGA   AGTGGTGGCC   TAACTACGGC   TACACTAGAA   4000
   4001   GGACAGTATT   TGGTATCTGC   GCTCTGCTGA   AGCCAGTTAC   CTTCGGAAAA   4050
   4051   AGAGTTGGTA   GCTCTTGATC   CGGCAAACAA   ACCACCGCTG   GTAGCGGTGG   4100
   4101   TTTTTTTGTT   TGCAAGCAGC   AGATTACGCG   CAGAAAAAAA   GGATCTCAAG   4150
   4151   AAGATCCTTT   GATCTTTTCT   ACGGGGTCTG   ACGCTCAGTG   GAACGAAAAC   4200
   4201   TCACGTTAAG   GGATTTTGGT   CATGAGATTA   TCAAAAAGGA   TCTTCACCTA   4250
   4251   GATCCTTTTA   AATTAAAAAT   GAAGTTTTAA   ATCAATCTAA   AGTATATATG   4300
   4301   AGTAAACTTG   GTCTGACAGT   TACCAATGCT   TAATCAGTGA   GGCACCTATC   4350
   4351   TCAGCGATCT   GTCTATTTCG   TTCATCCATA   GTTGCCTGAC   TCCCCGTCGT   4400
   4401   GTAGATAACT   ACGATACGGG   AGGGCTTACC   ATCTGGCCCC   AGTGCTGCAA   4450
   4451   TGATACCGCG   AGACCCACGC   TCACCGGCTC   CAGATTTATC   AGCAATAAAC   4500
   4501   CAGCCAGCCG   GAAGGGCCGA   GCGCAGAAGT   GGTCCTGCAA   CTTTATCCGC   4550
   4551   CTCCATCCAG   TCTATTAATT   GTTGCCGGGA   AGCTAGAGTA   AGTAGTTCGC   4600
   4601   CAGTTAATAG   TTTGCGCAAC   GTTGTTGCCA   TTGCTGCAGG   CATCGTGGTG   4650
   4651   TCACGCTCGT   CGTTTGGTAT   GGCTTCATTC   AGCTCCGGTT   CCCAACGATC   4700
   4701   AAGGCGAGTT   ACATGATCCC   CCATGTTGTG   CAAAAAAGCG   GTTAGCTCCT   4750
   4751   TCGGTCCTCC   GATCGTTGTC   AGAAGTAAGT   TGGCCGCAGT   GTTATCACTC   4800
   4801   ATGGTTATGG   CAGCACTGCA   TAATTCTCTT   ACTGTCATGC   CATCCGTAAG   4850
   4851   ATGCTTTTCT   GTGACTGGTG   AGTACTCAAC   CAAGTCATTC   TGAGAATAGT   4900
   4901   GTATGCGGCG   ACCGAGTTGC   TCTTGCCCGG   CGTCAACACG   GGATAATACC   4950
   4951   GCGCCACATA   GCAGAACTTT   AAAAGTGCTC   ATCATTGGAA   AACGTTCTTC   5000
   5001   GGGGCGAAAA   CTCTCAAGGA   TCTTACCGCT   GTTGAGATCC   AGTTCGATGT   5050
   5051   AACCCACTCG   TGCACCCAAC   TGATCTTCAG   CATCTTTTAC   TTTCACCAGC   5100
   5101   GTTTCTGGGT   GAGCAAAAAC   AGGAAGGCAA   AATGCCGCAA   AAAAGGGAAT   5150
   5151   AAGGGCGACA   CGGAAATGTT   GAATACTCAT ACTCTTCCTT   TTTCAATATT   5200
   5201   ATTGAAGCAT   TTATCAGGGT   TATTGTCTCA TGAGCGGATA   CATATTTGAA   5250
   5251   TGTATTTAGA   AAAATAAACA   AATAGGGGTT CCGCGCACAT   TTCCCCGAAA   5300
   5301   AGTGCCACCT   GACGTCTAAG   AAACCATTAT TATCATGACA   TTAACCTATA   5350
   5351   AAAATAGGCG   TATCACGAGG   CCCTTTCGT 5379
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