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p416 GpD质粒DNA说明书

发布时间：2024/10/24 15:43:50 阅读次数：181

CAT#:JW-1-274 常温运输及保存

本产品为p416 GpD质粒DNA干粉，是酿酒酵母表达载体，携带氨苄青霉素抗性基因。其图谱如下：

规格及成分
本产品使用塑料袋包装
成分            编号    规格    包装
p416 GpD质粒DNA干粉    1-274    2 mg    0.5 mL本色管
使用手册            1-274sc    1份    1份

运输及保存
常温运输，溶解后需-20℃保存，有效期两年

使用方法
使用前将本产品短暂离心后加入100 μL自备超纯水或TE缓冲液溶解，得到20 ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。

DNA序列

1        ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
61       TTAGGACGGA TCGCTTGCCT GTAACTTACA CGCGCCTCGT ATCTTTTAAT GATGGAATAA
121      TTTGGGAATT TACTCTGTGT TTATTTATTT TTATGTTTTG TATTTGGATT TTAGAAAGTA
181      AATAAAGAAG GTAGAAGAGT TACGGAATGA AGAAAAAAAA ATAAACAAAG GTTTAAAAAA
241      TTTCAACAAA AAGCGTACTT TACATATATA TTTATTAGAC AAGAAAAGCA GATTAAATAG
301      ATATACATTC GATTAACGAT AAGTAAAATG TAAAATCACA GGATTTTCGT GTGTGGTCTT
361      CTACACAGAC AAGATGAAAC AATTCGGCAT TAATACCTGA GAGCAGGAAG AGCAAGATAA
421      AAGGTAGTAT TTGTTGGCGA TCCCCCTAGA GTCTTTTACA TCTTCGGAAA ACAAAAACTA
481      TTTTTTCTTT AATTTCTTTT TTTACTTTCT ATTTTTAATT TATATATTTA TATTAAAAAA
541      TTTAAATTAT AATTATTTTT ATAGCACGTG ATGAAAAGGA CCCAGGTGGC ACTTTTCGGG
601      GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
661      TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
721      TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
781      CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
841      GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
901      GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG
961      ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
1021     ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
1081     CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
1141     CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
1201     GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG
1261     CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
1321     AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
1381     TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA
1441     TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
1501     GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
1561     TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
1621     TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
1681     TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
1741     CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
1801     TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
1861     GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC
1921     ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
1981     CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
2041     ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
2101     CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
2161     AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
2221     GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
2281     GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
2341     GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
2401     CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
2461     CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC
2521     CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
2581     GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TACCTCACTC
2641     ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCC TATGTTGTGT GGAATTGTGA
2701     GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA
2761     ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCAGTTTATC ATTATCAATA CTCGCCATTT
2821     CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA CTTTATTTAG TCAAAAAATT
2881     AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA GGGGGCGGGT TACACAGAAT
2941     ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG GCATCCACTA AATATAATGG
3001     AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC CCAGCACCAA AATATTGTTT
3061     TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG CAACTACAGA GAACAGGGGC
3121     ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT GCAACCTGCC TGGAGTAAAT
3181     GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA TTTTCTTACA CCTTCTATTA
3241     CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG GTTGAAACCA GTTCCCTGAA
3301     ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA GGTATTGATT GTAATTCTGT
3361     AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT TAGTCTTTTT TTTAGTTTTA
3421     AAACACCAGA ACTTAGTTTC GACGGATTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA
3481     TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGTCAT GTAATTAGTT ATGTCACGCT
3541     TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA GGAAGGAGTT AGACAACCTG
3601     AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT ATTAAGAACG TTATTTATAT
3661     TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC ATGTAACATT ATACTGAAAA
3721     CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCGGCCGG TACCCAATTC
3781     GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG
3841     GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG
3901     GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
3961     CGAATGGCGC GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
4021     CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
4081     CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
4141     GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
4201     ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
4261     CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
4321     TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
4381     ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT GATGCGGTAT
4441     TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAG GGTAATAACT GATATAATTA
4501     AATTGAAGCT CTAATTTGTG AGTTTAGTAT ACATGCATTT ACTTATAATA CAGTTTTTTA
4561     GTTTTGCTGG CCGCATCTTC TCAAATATGC TTCCCAGCCT GCTTTTCTGT AACGTTCACC
4621     CTCTACCTTA GCATCCCTTC CCTTTGCAAA TAGTCCTCTT CCAACAATAA TAATGTCAGA
4681     TCCTGTAGAG ACCACATCAT CCACGGTTCT ATACTGTTGA CCCAATGCGT CTCCCTTGTC
4741     ATCTAAACCC ACACCGGGTG TCATAATCAA CCAATCGTAA CCTTCATCTC TTCCACCCAT
4801     GTCTCTTTGA GCAATAAAGC CGATAACAAA ATCTTTGTCG CTCTTCGCAA TGTCAACAGT
4861     ACCCTTAGTA TATTCTCCAG TAGATAGGGA GCCCTTGCAT GACAATTCTG CTAACATCAA
4921     AAGGCCTCTA GGTTCCTTTG TTACTTCTTC TGCCGCCTGC TTCAAACCGC TAACAATACC
4981     TGGGCCCACC ACACCGTGTG CATTCGTAAT GTCTGCCCAT TCTGCTATTC TGTATACACC
5041     CGCAGAGTAC TGCAATTTGA CTGTATTACC AATGTCAGCA AATTTTCTGT CTTCGAAGAG
5101     TAAAAAATTG TACTTGGCGG ATAATGCCTT TAGCGGCTTA ACTGTGCCCT CCATGGAAAA
5161     ATCAGTCAAG ATATCCACAT GTGTTTTTAG TAAACAAATT TTGGGACCTA ATGCTTCAAC
5221     TAACTCCAGT AATTCCTTGG TGGTACGAAC ATCCAATGAA GCACACAAGT TTGTTTGCTT
5281     TTCGTGCATG ATATTAAATA GCTTGGCAGC AACAGGACTA GGATGAGTAG CAGCACGTTC
5341     CTTATATGTA GCTTTCGACA TGATTTATCT TCGTTTCCTG CAGGTTTTTG TTCTGTGCAG
5401     TTGGGTTAAG AATACTGGGC AATTTCATGT TTCTTCAACA CTACATATGC GTATATATAC
5461     CAATCTAAGT CTGTGCTCCT TCCTTCGTTC TTCCTTCTGT TCGGAGATTA CCGAATCAAA
5521     AAAATTTCAA AGAAACCGAA ATCAAAAAAA AGAATAAAAA AAAAATGATG AATTGAATTG
5581     AAAAGCTGTG GTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA
5641     GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC
5701     CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
5761     ATCACCGAAA CGCGCGA

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