关于商城

商城指南

支付方式

配送方式

售后服务

极威生物pCP20质粒DNA说明书

发布时间：2024/10/25 9:07:20 阅读次数：168

CAT#:JW-1-2372
低温运输，-20℃保存

产品及特点
本产品为pCP20质粒DNA，本质粒含有酵母的DNA重组酶基因（FLP，aka exo）及pSC101复制子，pCP20质粒DNA可用于基因敲除。本质粒携带Amp抗性基因，因结构特殊，本质粒在37℃时会失活。其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                编号    规格    包装
pCP20质粒DNA溶液     1-2372    2 mg    0.5 mL本色管
使用手册                        1-2372sc    1份    无

运输及保存
低温运输，-20℃保存，有效期两年

使用方法
使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。

DNA序列
1 11 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
151 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca
101 aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
151 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
201 gagtcagcaa caccttcttc acgaggcaga cctcagcgcc acaggtgcgg
251 ttgctggcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat
301 catatcgtca attattacct ccacggggag agcctgagca aactggcctc
351 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
401 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
451 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
501 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
551 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
601 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
651 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
701 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
751 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
801 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
851 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
901 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
951 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
1001 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
1051 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
1101 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
1151 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
1201 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
1251 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
1301 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
1351 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
1401 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
1451 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
1501 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg
1551 tgcgtaacgg caaaagcacc gccggacatc agcgcttgtt tcggcgtggg
1601 tatggtggca ggccccgtgg ccgggggact gttgggcgcc tgtagtgcca
1651 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac
1701 gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt
1751 cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt
1801 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa
1851 tactgcggaa ctgactaaag tagtgagtta tacacagggc tgggatctat
1901 tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt
1951 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt
2001 ctagcagaat ttacaagttt tccagcaaag gtctagcaga atttacagat
2051 acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc
2101 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct
2151 gaattagttg ttttcaaagc aaatgaacta gcgattagtc gctatgactt
2201 aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc
2251 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat
2301 aaccaatacg ttcagatgat gaacatcagt agggaaaatg cttatggtgt
2351 attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga
2401 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag
2451 ttctcaagcg aaaaattaga attagttttt agtgaagaga tattgcctta
2501 tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt
2551 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa
2601 ctaacacaaa agaaaactca caaggcaaat atagagatta gccttgatga
2651 atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc
2701 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc
2751 aatatgaaat tggtggttga taagcgaggc cgcccgactg atacgttgat
2801 tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga
2851 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca
2901 gattcctacc tacataacgg actaagaaaa acactacacg atgctttaac
2951 tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc
3001 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa
3051 cgaaccacac tagagaacat actggctaaa tacggaagga tctgaggttc
3101 ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt
3151 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccatatgcac
3201 agatgaaaac ggtgtaaaaa agatagatac atcagagctt ttacgagttt
3251 ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat
3301 gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca
3351 actagaacat gaaattgaac acctgagaca acttgttaca gctcaacagt
3401 cacacataga cagcctgaaa caggcgatgc tgcttatcga atcaaagctg
3451 ccgacaacac gggagccagt gacgcctccc gtggggaaaa aatcatggca
3501 attctggaag aaatagcgcc tgtttcgttt caggcaggtt atcagggagt
3551 gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca gcccgtaatg
3601 gtgatttacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt
3651 cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg
3701 acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat
3751 gctgtactga tttttttcga gaccgggcat cagtacacgc tcaaagctcg
3801 ccatcacttt ttcacgtcct cccggcggca gctccttctc cgcgaacgac
3851 agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc
3901 ccggacttct tccggattac cctgaagcac cgttgcgcct tcgcggttac
3951 gctccctccc cagcaggtaa tcaaccggac cactgccacc accttttccc
4001 ctggcatgaa atttaactat catcccgcgc cccctgttcc ctgacagcca
4051 gacgcagccg gcgcagctca tccccgatgg ccatcagtgc ggccaccacc
4101 tgaacccggt caccggaaga ccactgcccg ctgttcacct tacgggctgt
4151 ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg
4201 ccagtgtcgg cagttttccg gaacgggcaa ccggctcccc caggcagacc
4251 cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt caagtaaccg
4301 ggcatgttca tcatcagtaa cccgtattgt gagcatcctc tcgcgtttca
4351 tcggtatcat taccccatga acagaaatcc cccttacacg gaggcatcag
4401 tgactaaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
4451 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
4551 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
4601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4651 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
4701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
4751 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
4801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
4851 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
4901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
4951 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
5001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5051 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
5101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5151 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
5201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
5251 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
5301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
5351 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
5401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5451 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
5501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
5551 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
5601 cacgaggccc tttcgtcttc aagaatttta taaaccgtgg agcgggcaat
5651 actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
5701 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct
5751 ttcagctttg cttcctgtcg gccctcattc gtgcgctcta ggatcctcta
5801 cgccggacgc atcgtggccg gcatcaccgg cgctgaggtc tgcctcgtga
5851 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc
5901 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag
5951 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
6001 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac
6051 aaagccgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
6101 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
6151 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
6201 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
6251 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
6301 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
6351 gacgactgaa tccggtgaga atggcagaat aggaacttcg gaataggaac
6401 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
6451 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
6501 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
6551 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
6601 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac
6651 taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
6701 tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg
6751 agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat
6801 acgttgtcct ggccacggca gaagcacgct tatcgctcca atttcccaca
6851 acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa
6901 atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat
6951 aaggcgcatt tttcttcaaa gctttattgt acgatctgac taagttatct
7001 tttaataatt ggtattcctg tttattgctt gaagaattgc cggtcctatt
7051 tactcgtttt aggactggtt cagaattcct caaaaattca tccaaatata
7101 caagtggatc gatcctaccc cttgcgctaa agaagtatat gtgcctacta
7151 acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccagata
7201 cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc
7251 tgaatcttcc acaattgatg aaagtagcta ggaagaggaa ttggtataaa
7301 gtttttgttt ttgtaaatct cgaagtatac tcaaacgaat ttagtatttt
7351 ctcagtgatc tcccagatgc tttcaccctc acttagaagt gctttaagca
7401 tttttttact gtggctattt cccttatctg cttcttccga tgattcgaac
7451 tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg
7501 tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta
7551 atgaggcttc cagaattgtt gctttttgcg tcttgtattt aaactggagt
7601 gatttattga caatatcgaa actcagcgaa ttgcttatga tagtattata
7651 gctcatgaat gtggctctct tgattgctgt tccgttatgt gtaatcatcc
7701 aacataaata ggttagttca gcagcacata atgctatttt ctcacctgaa
7751 ggtctttcaa acctttccac aaactgacga acaagcacct taggtggtgt
7801 tttacataat atatcaaatt gtggcataca acctccttag tacatgcaac
7851 cattatcacc gccagaggta aaatagtcaa cacgcacggt gttagatatt
7901 tatcccttgc ggtgatagat ttaacgtatg agcacaaaaa agaaaccatt
7951 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa
8001 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg
8051 gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt
8101 aaatgcttat aacgccgcat tgcttacaaa aattctcaaa gttagcgttg
8151 aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg
8201 gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
8251 tcatgttcag gcagggatgt tctcacctaa gcttagaacc tttaccaaag
8301 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca
8351 ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa
8401 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg
8451 ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc
8501 ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
8551 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga
8601 aagttatcgc tagtcagtgg cctgaagaga cgtttggctg atcggcaagg
8651 tgttctggtc ggcgcatagc tgataacaat tgagcaagaa tctgcatttc
8701 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
8751 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
8801 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
8851 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
8901 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
8951 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
9001 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
9051 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
9101 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
9151 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
9201 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
9251 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
9301 tttatcttgt gcaatgtaac atcagagatt tt 9332 cagtttt attgt

关联产品
大肠杆菌DH5α感受态细胞