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磷酸二酯酶Ⅱ10U¥ 1400100 瓶
英文名称:Phosphodiesterase II from bovine spleen;3′-Exonuclease;Spleen exonuclease;Spleen phosphodiesterase
其他名称:磷酸二脂酶;磷酸二酯酶
CAS号:9068-54-6
级别:BR
Specific activity:≥1.2u/mg protein
活力定义:One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
产品描述:Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
性状(以下信息仅供参考):粉末
用途:本品仅供科研,不得用于其它用途
保存:-20℃
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