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名称 | HTR-8/Svneo(人绒毛膜滋养层细胞) |
别称 | HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn |
年龄(性别) | 女;6-12周胚胎 |
组织来源 | 胎盘 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. |
生物安全等级 | 2 |
生长培养基 | RPMI-1640(PM150110)+5% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:3-1:5 |
推荐换液频率 | 2-3次/周 |
冻存条件 | 冻存液:50%基础培养基+40%胎牛血清+10%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
致瘤性 | No (tested in soft agar and nude mice). |
抗原表达情况 | cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha |
保藏机构 | ATCC; CRL-3271 |
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