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名称 | bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) |
别称 | bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3 |
年龄(性别) | 6周龄 |
组织来源 | 大脑皮层 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen |
生物安全等级 | 1 |
生长培养基 | DMEM高糖(PM150210)+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2-3天/次 |
倍增时间 | ~24-32 hours |
冻存条件 | 冻存液:50%基础培养基+40%胎牛血清+10%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
抗原表达情况 | ICAM-1 +; VCAM-1 +; MAdCAM-1 + |
基因表达情况 | von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's |
保藏机构 | ATCC; CRL-2299BCRC; 60515ECACC; 96091929 |