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产品名称
起订量
单价(含增值税)
库存(PCS)
起订数量
产品概述
产品信息
| 别名 | Sonic Hedgehog Protein; SHH; HHG-1; SHH |
| 物种 | Mouse |
| 表达宿主 | E.coli |
| 序列信息 | Met1-Gly198 |
| 检索号 | Q62226 |
| 分子量 | 19.8 kDa |
| 表观分子量 | 20 kDa |
| 生物活性 | Measured by its ability to induce alkaline phosphatase production by C3H10T1/ 2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.The ED50 for this effect is typically 5-40 μg/ mL. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg as determined by LAL test. |
| 保存 | Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| 运输 | In general, recombinant Proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant Proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. |
| 复融 | Please refer to it for detailed information. |
背景介绍
Mouse Sonic Hedgehog Homolog (SHH) belongs to a three- Protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog Proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh-N, respectively.
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