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Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser15GV-P00572-10ug¥ 844.001
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Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser15GV-P00572-50ug¥ 1800.001
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Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser15GV-P00572-500ug¥ 6520.001
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Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser15GV-P00572-1mg¥ 8800.001
产品简介
中文名称 | Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser155)(Q65I,C96S,N111G) |
Known as | Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB |
Derived from | E.coli |
Purity | Greater than 90% as determined by reducing SDS-PAGE. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl, pH7.5. |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxin | Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test. |
Background | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
规格 | 10ug50ug500ug1mg |
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
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