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产品简介

基因名	OTUB1
英文全称	OTU domain, ubiquitin aldehyde binding 1
别称	HSPC263;OTB1;OTU1
中文名称	OTU域,泛素醛结合1
Uniprot	Q96FW1
GeneID	55611
交叉反应(推测)	Human Mouse Rat
氨基酸大小	31kDa
表达氨基酸	1-271aa
Background	Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation.Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen.Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy.Isoform 1 destabilizes RNF128, leading to prevent anergy.In contrast, isoform 2 stabilizes RNF128 and promotes anergy.Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128.Deubiquitinates estrogen receptor alpha (ESR1).Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains.Not able to cleave di-ubiquitin.Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites.Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks.Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1.The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.
Application	Immunology research
Derived from	E.coli
Purification	NI-NTA affinity purification
Formulation	Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping	The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage	Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution	Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity	Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin	/
Bioactive	Unknown. (Ban't be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格	10ug50ug100ug1mg