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产品简介

基因名	APEX1
英文全称	APEX nuclease (multifunctional DNA repair enzyme) 1
别称	APEN；AP endonuclease 1；APE-1；APE；APE1；APEX；APX；HAP1；REF1
中文名称	APEX核酸酶1
Uniprot	P27695
GeneID	328
交叉反应(推测)	Human Mouse Rat (Bovine)
氨基酸大小	35kDa
表达氨基酸	1-319aa
Background	Multifunctional protein that plays a central role in the cellular response to oxidative stress.The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors.Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules.Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER.Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks.May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB.Plays a role in the protection from granzymes-mediated cellular repair leading to cell death.Also involved in the DNA cleavage step of class switch recombination (CSR).On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR.Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs).Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression.Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance.Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism.Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD).In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression.Associates, together with YBX1, on the MDR1 promoter.Together with NPM1, associates with rRNA.Binds DNA and RNA.
Application	Immunology research
Derived from	E.coli
Purification	NI-NTA affinity purification
Formulation	Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping	The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage	Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution	Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity	Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin	/
Bioactive	Unknown. (Ban't be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格	10ug50ug100ug1mg