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产品简介
英文名称 | Anti-Flag tag Monoclonal Antibody |
别名 | DDDDK Tag;DYKDDDDK Tag |
稀释比例 | WB 1:10000-15000. IP 1:100-300. IF 1:500-3000. |
保存 | Store at -20°C. Avoid freeze / thaw cycles. |
储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
免疫原 | A synthetic peptide coupled to KLH |
亚型 | IgG1 |
性状 | 液体 |
纯化方法 | Affinity purification |
克隆号 | 2C5 |
背景资料 | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence. |
Public Immunogen Range | A synthetic peptide coupled to KLH |
克隆类型 | Monoclonal Antibody |
应用 | WBIFIP |
规格 | 50ul100ul |
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
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